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IF (Immunofluorescence)
(Staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF7232 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
Rabbit B-Raf Polyclonal Antibody | anti-B-Raf antibody
Phospho-B-Raf (Ser729) Antibody
Gene Names
BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
Reactivity
Human , Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
B-Raf, Antibody; Phospho-B-Raf (Ser729) Antibody; FLJ95109; 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; B Raf proto oncogene, serine/threonine kinase; B RAF1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF 1; BRAF; BRAF_HUMAN; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral (v-raf) oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; NS7; Oncogen BRAF; oncogene BRAF1; p94; Proto-oncogene B-Raf; Proto-oncogene c-Rmil; RAFB 1; RAFB1; RMIL; Serine/threonine-protein kinase B-raf; v raf murine sarcoma viral oncogene homolog B; v raf murine sarcoma viral oncogene homolog B1; v-Raf murine sarcoma viral oncogene homolog B1; anti-B-Raf antibody
Host
Rabbit
Reactivity
Human , Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-B-Raf (Ser729) Antibody detects endogenous levels of B-Raf only when phosphorylated at Ser729.
Purity/Purification
Peptide affinity purification
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
766
Applicable Applications for anti-B-Raf antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human B-Raf around the phosphorylation site of Ser729.
RRID
AB_2843672
Subcellular Location
Plasma Membrane; Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(Staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF7232 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
IF (Immunofluorescence)
(Staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF7232 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-B-Raf (Ser729) in lysates of PC-12?, using Phospho-B-Raf (Ser729) Antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-B-Raf (Ser729) in lysates of PC-12?, using Phospho-B-Raf (Ser729) Antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemisry)
(At 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-B-Raf antibody
Description: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
84.4 kDa
NCBI Official Full Name
serine/threonine-protein kinase B-raf isoform 1
NCBI Official Synonym Full Names
B-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol
BRAF
NCBI Official Synonym Symbols
NS7; B-raf; BRAF1; RAFB1; B-RAF1
NCBI Protein Information
serine/threonine-protein kinase B-raf
UniProt Protein Name
Serine/threonine-protein kinase B-raf
UniProt Gene Name
BRAF
UniProt Synonym Gene Names
BRAF1; RAFB1
UniProt Entry Name
BRAF_HUMAN
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Product Notes
The B-Raf braf (Catalog #AAA328389) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-B-Raf (Ser729) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's B-Raf can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the B-Raf braf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B-Raf, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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