WB (Western Blot)
(Western blot analysis of BACE using HeLa whole cell lysates)
Rabbit BACE Polyclonal Antibody | anti-BACE antibody
BACE Antibody
Gene Names
BACE1; ASP2; BACE; HSPC104
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
BACE, Antibody; BACE Antibody; APP beta secretase; Asp 2; ASP2; Aspartyl protease 2; BACE 1; BACE; BACE1; BACE1_HUMAN; Beta secretase 1; Beta secretase; Beta site amyloid beta A4 precursor protein cleaving enzyme; Beta site amyloid precursor protein cleaving enzyme 1; Beta site amyloid precursor protein cleaving enzyme; Beta site APP cleaving enzyme 1; Beta site APP cleaving enzyme; Beta-secretase 1; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; FLJ90568; HSPC104; Memapsin 2; Memapsin-2; Memapsin2; Membrane associated aspartic protease 2; Membrane-associated aspartic protease 2; Transmembrane aspartic proteinase Asp2; anti-BACE antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
BACE antibody detects endogenous levels of total BACE
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
376
Applicable Applications for anti-BACE antibody
ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human BACE
Subcellular Location
Membrane. Golgi Apparatus > Trans-Golgi network. Endoplasmic Reticulum. Endosome. Cell Surface. Predominantly localized to the later golgi/trans-golgi network (TGN) and minimally detectable in the early golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
Tissue Specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (93%), Bovine (93%), Horse (93%), Sheep (93%), Rabbit (93%), Dog (93%), Chicken (87%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of BACE using HeLa whole cell lysates)
WB (Western Blot)
(Western blot analysis of BACE using HeLa whole cell lysates)
WB (Western Blot)
(Western blot analysis of extracts from mouse kidney, mouse heart, using BACE Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse kidney, mouse heart, using BACE Antibody.)
Related Product Information for anti-BACE antibody
Function: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
Subunit Structure: Monomer. Interacts with GGA1, GGA2 and GGA3. Interacts with RTN3 and RTN4. Interacts with SNX6. Interacts with PCSK9. Interacts with NAT8 and NAT8B.
Post-translational Modifications: N-Glycosylated. Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein. Palmitoylation mediates lipid raft localization.
Similarity: The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. Belongs to the peptidase A1 family.
Subunit Structure: Monomer. Interacts with GGA1, GGA2 and GGA3. Interacts with RTN3 and RTN4. Interacts with SNX6. Interacts with PCSK9. Interacts with NAT8 and NAT8B.
Post-translational Modifications: N-Glycosylated. Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein. Palmitoylation mediates lipid raft localization.
Similarity: The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. Belongs to the peptidase A1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 70 kDa
Predicted: 56 kDa
Predicted: 56 kDa
NCBI Official Full Name
beta-secretase 1 isoform F
NCBI Official Synonym Full Names
beta-secretase 1
NCBI Official Symbol
BACE1
NCBI Official Synonym Symbols
ASP2; BACE; HSPC104
NCBI Protein Information
beta-secretase 1
UniProt Protein Name
Beta-secretase 1
UniProt Gene Name
BACE1
UniProt Synonym Gene Names
BACE; KIAA1149; ASP2; Asp 2; Beta-site APP cleaving enzyme 1
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Product Notes
The BACE bace1 (Catalog #AAA327248) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BACE Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BACE can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the BACE bace1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BACE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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