WB (Western Blot)
(Anti-BACH1 Antibody - Western Blot. Western blot of Affinity Purified anti-BACH1 antibody shows detection of a band at ~105 kD (lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (arrowhead). Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction. Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
Rabbit anti-Human BACH1 Polyclonal Antibody | anti-BACH1 antibody
Rabbit Polyclonal to Human BACH1
Gene Names
BACH1; BACH-1; BTBD24
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
BACH1, Antibody; Rabbit Polyclonal to Human BACH1; Anti-BACH1 Antibody (aa92-104) IHC-plus; BACH1; BTB and CNC homolog 1; HA2303; BACH-1; Human BACH1; anti-BACH1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Amino acids 92-104 of isoform 1 of human BACH1 protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
736
Applicable Applications for anti-BACH1 antibody
ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Recombinant human BACH1.
Immunogen Type
Recombinant protein
Immunogen
BACH1 antibody was raised against recombinant human BACH1.
Antigen Modification
aa92-104
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
WB (Western Blot)
(Anti-BACH1 Antibody - Western Blot. Western blot of Affinity Purified anti-BACH1 antibody shows detection of a band at ~105 kD (lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (arrowhead). Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction. Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
WB (Western Blot)
(Anti-BACH1 Antibody - Western Blot. Western blot of Affinity Purified anti-BACH1 antibody shows detection of a band at ~105 kD (lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (arrowhead). Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction. Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
IHC (Immunohistochemistry)
(Anti-BACH1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-BACH1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
81,958 Da
NCBI Official Full Name
transcription regulator protein BACH1
NCBI Official Synonym Full Names
BTB and CNC homology 1, basic leucine zipper transcription factor 1
NCBI Official Symbol
BACH1
NCBI Official Synonym Symbols
BACH-1; BTBD24
NCBI Protein Information
transcription regulator protein BACH1; basic region leucine zipper transcriptional regulator BACH1
UniProt Protein Name
Transcription regulator protein BACH1
UniProt Gene Name
BACH1
UniProt Entry Name
BACH1_HUMAN
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Product Notes
The BACH1 bach1 (Catalog #AAA50752) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human BACH1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BACH1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the BACH1 bach1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BACH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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