WB (Western Blot)
(Western blot analysis of BACH1/BRIP1 in lysates of Jurkat , using BACH1/BRIP1 Antibody(AF4789).)
Rabbit BACH1/BRIP1 Polyclonal Antibody | anti-BACH1/BRIP1 antibody
BACH1/BRIP1 Antibody
Gene Names
BRIP1; OF; BACH1; FANCJ
Reactivity
Human, MousePredicted Reactivity: Pig (100%), Bovine (100%), Dog (92%), Chicken (91%)
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
BACH1/BRIP1, Antibody; BACH1/BRIP1 Antibody; ATP dependent RNA helicase BRIP1; ATP-dependent RNA helicase BRIP1; BACH 1; BRAC 1 Associated C Terminal Helicase 1; BRCA 1 Interacting Protein 1; BRCA1 binding helicase like protein BACH1; BRCA1 interacting protein C terminal helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-interacting protein 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1/BRCA2 associated helicase 1; BRIP 1; BRIP1; FANCJ; FANCJ_HUMAN; Fanconi anemia group J protein; FLJ90232; MGC126521; MGC126523; OF; Protein FACJ; anti-BACH1/BRIP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted Reactivity: Pig (100%), Bovine (100%), Dog (92%), Chicken (91%)
Predicted Reactivity: Pig (100%), Bovine (100%), Dog (92%), Chicken (91%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
BACH1/BRIP1 Antibody detects endogenous levels of total BACH1/BRIP1.
Tissue Specificity: Ubiquitously expressed, with highest levels in testis.
Tissue Specificity: Ubiquitously expressed, with highest levels in testis.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-BACH1/BRIP1 antibody
ELISA (Peptide ELISA), WB (Western Blot)
Immunogen
A synthesized peptide derived from human BACH1/BRIP1, corresponding to a region within C-terminal amino acids.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated. Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling.
Subunit Structure
Binds directly to the BRCT domains of BRCA1. Interacts with the CIA complex components CIAO1, CIAO2B and MMS19.
Similarity
4Fe-4S iron-sulfur-binding is required for helicase activity.Belongs to the DEAD box helicase family. DEAH subfamily.
Subcellular Location
Nucleus. Cytoplasm.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of BACH1/BRIP1 in lysates of Jurkat , using BACH1/BRIP1 Antibody(AF4789).)
WB (Western Blot)
(Western blot analysis of BACH1/BRIP1 in lysates of Jurkat , using BACH1/BRIP1 Antibody(AF4789).)
WB (Western Blot)
(Western blot analysis of extracts from MCF7, using BACH1/BRIP1 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from MCF7, using BACH1/BRIP1 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using BACH1/BRIP1 Antibody.Lane 1: A2780 cells, blocked with antigen-specific peptides,Lane 2: A2780 cells,Lane 3: HepG2 cells,Lane 4: RAW264.7 cells.7.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using BACH1/BRIP1 Antibody.Lane 1: A2780 cells, blocked with antigen-specific peptides,Lane 2: A2780 cells,Lane 3: HepG2 cells,Lane 4: RAW264.7 cells.7.)
Related Product Information for anti-BACH1/BRIP1 antibody
DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
112,476 Da
NCBI Official Full Name
Fanconi anemia group J protein
NCBI Official Synonym Full Names
BRCA1 interacting protein C-terminal helicase 1
NCBI Official Symbol
BRIP1
NCBI Official Synonym Symbols
OF; BACH1; FANCJ
NCBI Protein Information
Fanconi anemia group J protein; ATP-dependent RNA helicase BRIP1; BRCA1/BRCA2-associated helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-binding helicase-like protein BACH1
UniProt Protein Name
Fanconi anemia group J protein
UniProt Gene Name
BRIP1
UniProt Synonym Gene Names
BACH1; FANCJ; Protein FACJ; BRCA1-interacting protein 1
UniProt Entry Name
FANCJ_HUMAN
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Product Notes
The BACH1/BRIP1 brip1 (Catalog #AAA329569) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BACH1/BRIP1 Antibody reacts with Human, Mouse Predicted Reactivity: Pig (100%), Bovine (100%), Dog (92%), Chicken (91%) and may cross-react with other species as described in the data sheet. AAA Biotech's BACH1/BRIP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), WB (Western Blot). Researchers should empirically determine the suitability of the BACH1/BRIP1 brip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BACH1/BRIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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