WB (Western Blot)
(Detection of human BRIP1/BACH1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-BRIP1/BACH1 antibody AAA211023 (lot AAA211023-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
Rabbit anti-Human BRIP1/BACH1 Polyclonal Antibody | anti-BRIP1/BACH1 antibody
Rabbit anti-BRIP1/BACH1 Antibody, Affinity Purified
Gene Names
BRIP1; OF; BACH1; FANCJ
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
BRIP1/BACH1, Antibody; Rabbit anti-BRIP1/BACH1 Antibody, Affinity Purified; FANCJ; Protein FACJ; Fanconi anemia group J protein; BRCA1-interacting protein C-terminal helicase 1; BRCA1-interacting protein 1; BRCA1-associated C-terminal helicase 1; BRCA1/BRCA2-associated helicase 1; BACH1; ATP-dependent RNA helicase BRIP1; OF; BRCA1-binding helicase-like protein BACH1; BRCA1 interacting protein C-terminal helicase 1; anti-BRIP1/BACH1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-BRIP1/BACH1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1199 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human BRIP1/BACH1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-BRIP1/BACH1 antibody AAA211023 (lot AAA211023-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human BRIP1/BACH1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-BRIP1/BACH1 antibody AAA211023 (lot AAA211023-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human BRIP1/BACH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-BRIP1/BACH1 antibody AAA211023 (lot AAA211023-1) used for IP at 3 ug/mg lysate. BRIP1/BACH1 was also immunoprecipitated using rabbit anti-BRIP1/BACH1 antibody which is to an upstream epitope, at 3 ug/mg lysate. For blotting immunoprecipitated BRIP1/BACH1, AAA211023 was used at 1 ug/ml.Detection: Chemiluminescence with exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human BRIP1/BACH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-BRIP1/BACH1 antibody AAA211023 (lot AAA211023-1) used for IP at 3 ug/mg lysate. BRIP1/BACH1 was also immunoprecipitated using rabbit anti-BRIP1/BACH1 antibody which is to an upstream epitope, at 3 ug/mg lysate. For blotting immunoprecipitated BRIP1/BACH1, AAA211023 was used at 1 ug/ml.Detection: Chemiluminescence with exposure time of 10 seconds.)
Related Product Information for anti-BRIP1/BACH1 antibody
BRCA1-interacting protein 1 (BRIP1) is a RecQ DNA helicase that associates with BRCA1 and functions in double-strand break repair and the maintenance of chromosomal stability. BRIP1 has been found to be deficient in Fanconi anemia (FA), a chromosomal instability syndrome associated with a strong predisposition to cancer.
Product Categories/Family for anti-BRIP1/BACH1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
112,476 Da
NCBI Official Full Name
Homo sapiens BRCA1 interacting protein C-terminal helicase 1 (BRIP1), mRNA
NCBI Official Synonym Full Names
BRCA1 interacting protein C-terminal helicase 1
NCBI Official Symbol
BRIP1
NCBI Official Synonym Symbols
OF; BACH1; FANCJ
NCBI Protein Information
Fanconi anemia group J protein; ATP-dependent RNA helicase BRIP1; BRCA1-associated C-terminal helicase 1; BRCA1-binding helicase-like protein BACH1; BRCA1/BRCA2-associated helicase 1
UniProt Protein Name
Fanconi anemia group J protein
UniProt Gene Name
BRIP1
UniProt Synonym Gene Names
BACH1; FANCJ; Protein FACJ; BRCA1-interacting protein 1
UniProt Entry Name
FANCJ_HUMAN
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Product Notes
The BRIP1/BACH1 brip1 (Catalog #AAA211023) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BRIP1/BACH1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRIP1/BACH1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the BRIP1/BACH1 brip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRIP1/BACH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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