Rabbit BAHD1 Polyclonal Antibody | anti-BAHD1 antibody

BAHD1 Antibody (C-term)

Cat. Num. AAA284907

• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: BAHD1, Antibody; BAHD1 Antibody (C-term); Bromo adjacent homology domain-containing 1 protein; BAH domain-containing protein 1; BAHD1; KIAA0945; anti-BAHD1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This BAHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 750-777 amino acids from the C-terminal region of human BAHD1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 750-777
• Sequence Length: 777

• Applicable Applications for anti-BAHD1 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(BAHD1 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with BAHD1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of BAHD1 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). BAHD1 (arrow) was detected using the purified Pab.)

Related Product Information for anti-BAHD1 antibody

BAHD1 is a heterochromatin protein that acts as a transcription repressor and has the ability to promote the formation of large heterochromatic domains. It may act by recruiting heterochromatin proteins such as CBX5 (HP1 alpha), HDAC5 and MBD1. It represses IGF2 expression by binding to its CpG-rich P3 promoter and recruiting heterochromatin proteins.

References

Mayya V.,et.al., Sci. Signal. 2:RA46-RA46(2009).
Dephoure N., et.al., Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 139394604
• NCBI GeneID: 22893
• NCBI Accession #: NP_055767.3
• NCBI GenBank Nucleotide #: NM_014952.4
• UniProt Accession #: Q8TBE0; Q8NDF7; Q9Y2F4
• Molecular Weight: 84652
• NCBI Official Full Name: bromo adjacent homology domain-containing 1 protein isoform a
• NCBI Official Synonym Full Names: bromo adjacent homology domain containing 1
• NCBI Official Symbol: BAHD1
• NCBI Protein Information: bromo adjacent homology domain-containing 1 protein
• UniProt Protein Name: Bromo adjacent homology domain-containing 1 protein
• UniProt Gene Name: BAHD1
• UniProt Synonym Gene Names: KIAA0945; BAH domain-containing protein 1
• UniProt Entry Name: BAHD1_HUMAN