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WB (Western Blot)
(Western blot analysis of extracts from HepG2, rat heart, using BCAR3 Antibody.)
Rabbit anti-Human, Rat BCAR3 Polyclonal Antibody | anti-BCAR3 antibody
BCAR3 Antibody
Gene Names
BCAR3; NSP2; AND-34; SH2D3B
Reactivity
Human, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
BCAR3, Antibody; BCAR3 Antibody; BCAR 3; BCAR3; BCAR3_HUMAN; Breast cancer anti estrogen resistance 3; Breast cancer anti estrogen resistance protein 3; Breast cancer anti-estrogen resistance protein 3; Breast cancer antiestrogen resistance 3; dJ1033H22.2; dJ1033H22.2 breast cancer anti estrogen resistance 3; dJ1033H22.2 breast cancer antiestrogen resistance 3; KIAA0554; Novel SH2 containing protein 2; Novel SH2-containing protein 2; NSP 2; NSP2; SH2 containing protein Nsp2; SH2 domain containing protein 3B; SH2 domain-containing protein 3B; SH2D3B; anti-BCAR3 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
BCAR3 antibody detects endogenous levels of BCAR3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
825
Applicable Applications for anti-BCAR3 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human BCAR3
Tissue Specificity
Ubiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (90%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from HepG2, rat heart, using BCAR3 Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2, rat heart, using BCAR3 Antibody.)
IF (Immunofluorescence)
(AAA321099 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA321099 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis on HeLa cell lysate using BCAR3 Antibody, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis on HeLa cell lysate using BCAR3 Antibody, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry)
(AAA321099 at 1/100 staining human Breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA321099 at 1/100 staining human Breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-BCAR3 antibody
Description: A ubiquitously expressed regulator of R-Ras activity that has been implicated in breast cancer. A GDP exchange factor whose overexpression confers antiestrogen resistance, activates Rac, PAK1, and the cyclin D1. Can accelerate the degradation of IRS-1, thus regulating signaling through the insulin receptor. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins. Up-regulated by interleukin-1-alpha and TNF-beta in thymus cortical reticular cell lines (mouse).Two alternatively spliced human isoforms have been described.
Function: May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.
Subunit Structure: Interacts with BCAR1, NEDD9, PTK2/FAK1 and PTPN1.
Function: May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.
Subunit Structure: Interacts with BCAR1, NEDD9, PTK2/FAK1 and PTPN1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 92 kDa
Predicted: 93 kDa
Predicted: 93 kDa
NCBI Official Full Name
breast cancer anti-estrogen resistance protein 3 isoform 1
NCBI Official Synonym Full Names
BCAR3, NSP family adaptor protein
NCBI Official Symbol
BCAR3
NCBI Official Synonym Symbols
NSP2; AND-34; SH2D3B
NCBI Protein Information
breast cancer anti-estrogen resistance protein 3
UniProt Protein Name
Breast cancer anti-estrogen resistance protein 3
UniProt Gene Name
BCAR3
UniProt Synonym Gene Names
NSP2; SH2D3B
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Product Notes
The BCAR3 bcar3 (Catalog #AAA321099) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BCAR3 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BCAR3 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the BCAR3 bcar3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCAR3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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