Rabbit BDNF Polyclonal Antibody | anti-BDNF antibody

BDNF Antibody (C-term)

Cat. Num. AAA288741

• Gene Names: BDNF; ANON2; BULN2
• Reactivity: Human; Predicted Reactivity: Bovine, Horse, Mouse, Pig, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: BDNF, Antibody; BDNF Antibody (C-term); Brain-derived neurotrophic factor; BDNF; Abrineurin; anti-BDNF antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Bovine, Horse, Mouse, Pig, Rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This BDNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the C-terminal region of human BDNF.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 206-236
• Sequence Length: 247

• Applicable Applications for anti-BDNF antibody: FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Function: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
• Cellular location: Secreted
• Tissue location: Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expresed in heart, lung , skeletal muscle, testis, prostate and placenta.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of CEM cells using BDNF Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of BDNF Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human brain reacted with BDNF Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Application Data

(All lanes : Anti-BDNF Antibody (C-term) at 1:1000 dilution Lane 1: human cerebellum lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST)

Related Product Information for anti-BDNF antibody

BDNF is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this protein is reduced in both Alzheimer's and Huntington disease patients. This protein may play a role in the regulation of stress response and in the biology of mood disorders.

Product Categories/Family for anti-BDNF antibody

Cardiovascular; Metabolism; Neuroscience; Signal Transduction

References

Jiang,H., Clin. Chim. Acta 400 (1-2), 3-7 (2009)
Liu,L., Psychiatr. Genet. 18 (6), 267-274 (2008)
Dmitrzak-Weglarz,M., Pharmacogenomics 9 (11), 1595-1603 (2008)

NCBI and Uniprot Product Information

• NCBI GI #: 219842288
• NCBI GeneID: 627
• NCBI Accession #: NP_001137277.1; NP_001137278.1; NP_001137279.1; NP_001137280.1; NP_001137281.1; NP_001137282.1; NP_001137283.1; NP_001137284.1; NP_001137285.1; NP_001137286.1; NP_001137288.1; NP_001700.2; NP_733927.1; NP_733928.1
• NCBI GenBank Nucleotide #: NM_001143805.1
• UniProt Accession #: P23560; Q598Q1; Q6DN19; Q6YNR2; Q6YNR3; Q9BYY7; Q9UC24; A7LA85; A7LA92; D3DQZ2
• NCBI Official Full Name: brain-derived neurotrophic factor isoform a preproprotein
• NCBI Official Synonym Full Names: brain-derived neurotrophic factor
• NCBI Official Symbol: BDNF
• NCBI Official Synonym Symbols: ANON2; BULN2
• NCBI Protein Information: brain-derived neurotrophic factor
• UniProt Protein Name: Brain-derived neurotrophic factor
• UniProt Gene Name: BDNF
• UniProt Synonym Gene Names: BDNF
• UniProt Entry Name: BDNF_HUMAN