IP (Immunoprecipitation)
(ACTB was immunoprecipitated using:; Lane A:0.5 mg MOLT-4 Whole Cell Lysate; Lane B:0.5 mg Raji Whole Cell Lysate; 4 uL anti-ACTB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-ACTB rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 43 kDa; Observed band size :43 kDa)
Rabbit anti-Human beta-Actin Polyclonal Antibody | anti-ACTB antibody
beta-Actin Antibody, Rabbit PAb, Antigen Affinity Purified
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Synonyms
beta-Actin, Antibody; beta-Actin Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-BRWS1 Antibody; Anti-PS1TP5BP1 Antibody; anti-ACTB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human beta-Actin/ACTB
Applicable Applications for anti-ACTB antibody
IP (Immunoprecipitation), WB (Western Blot)
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the human beta actin., and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(ACTB was immunoprecipitated using:; Lane A:0.5 mg MOLT-4 Whole Cell Lysate; Lane B:0.5 mg Raji Whole Cell Lysate; 4 uL anti-ACTB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-ACTB rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 43 kDa; Observed band size :43 kDa)
IP (Immunoprecipitation)
(ACTB was immunoprecipitated using:; Lane A:0.5 mg MOLT-4 Whole Cell Lysate; Lane B:0.5 mg Raji Whole Cell Lysate; 4 uL anti-ACTB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-ACTB rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 43 kDa; Observed band size :43 kDa)
WB (Western Blot)
(Anti-beta-Actin rabbit polyclonal antibody at 1:1000 dilution; Lane A: MOLT-4 Whole Cell Lysate; Lane B: Raji Whole Cell Lysate; Lane C: PC3 Whole Cell Lysate; Lane D: PC12 Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:42 kDa; Observed band size:43 kDa)
WB (Western Blot)
(Anti-beta-Actin rabbit polyclonal antibody at 1:1000 dilution; Lane A: MOLT-4 Whole Cell Lysate; Lane B: Raji Whole Cell Lysate; Lane C: PC3 Whole Cell Lysate; Lane D: PC12 Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:42 kDa; Observed band size:43 kDa)
Related Product Information for anti-ACTB antibody
ACTB (Actin Beta) is a Protein Coding gene. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is ACTG1. TUBB, HPRT and ACTB were the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood.
Product Categories/Family for anti-ACTB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
375
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
NCBI Protein Information
actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN
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Product Notes
The ACTB actb (Catalog #AAA258622) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The beta-Actin Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Actin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Actin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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