ICC (Immunocytochemistry)
(Immunocytochemical labeling of phosphorylated beta-Catenin in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal beta-Catenin (CM1181) or rabbit polyclonal beta-Catenin (Tyr-86) or beta-Catenin (Y654) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)
Rabbit beta-Catenin Polyclonal Antibody | anti-CTNNB1 antibody
beta-Catenin (Tyr-654)[gamma-Catenin (Tyr-644)], phospho-specific Antibody
Gene Names
CTNNB1; CTNNB; MRD19; armadillo
Reactivity
Human, Rat, Mouse, Chicken, Fish
Applications
Immunocytochemistry, ELISA, Western Blot
Synonyms
beta-Catenin, Antibody; beta-Catenin (Tyr-654)[gamma-Catenin (Tyr-644)], phospho-specific Antibody; beta-Catenin (Tyr-654)[gamma-Catenin (Tyr-644)], phospho-specific; Catenin 2 Tyr-654; anti-CTNNB1 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Chicken, Fish
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to phospho-beta-Catenin (Tyr-86) peptide before affinity purification using phospho-beta-Catenin (Tyr-654) peptide (without carrier). The antibody detects 84 and 88kDa* proteins corresponding to the molecular mass of gamma-Catenin and beta-Catenin, respectively, on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but does not detect these proteins in control cells.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-CTNNB1 antibody
ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Phospho-beta-Catenin (Tyr-654) synthetic peptide corresponds to amino acid residues around tyrosine 654 of human beta-Catenin. This peptide sequence is highly conserved in rat, mouse, chicken, and frog beta-Catenins, and has high homology to the conserved site in gamma-Catenin (Tyr-644).
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of phosphorylated beta-Catenin in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal beta-Catenin (CM1181) or rabbit polyclonal beta-Catenin (Tyr-86) or beta-Catenin (Y654) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of phosphorylated beta-Catenin in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal beta-Catenin (CM1181) or rabbit polyclonal beta-Catenin (Tyr-86) or beta-Catenin (Y654) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)
WB (Western Blot)
(Western blot analysis of A431 cells stimulated with pervanadate (1mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-beta-Catenin (CM1181) (lanes 1 & 2), anti-beta-Catenin (Tyr-654) (CP4021) (lanes 3 & 4), or anti-gamma-Catenin (CM1111) (lanes 5 & 6).)
WB (Western Blot)
(Western blot analysis of A431 cells stimulated with pervanadate (1mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-beta-Catenin (CM1181) (lanes 1 & 2), anti-beta-Catenin (Tyr-654) (CP4021) (lanes 3 & 4), or anti-gamma-Catenin (CM1111) (lanes 5 & 6).)
Related Product Information for anti-CTNNB1 antibody
beta-Catenin is a 92kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of beta-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn Kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between alpha- and beta-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of beta-Catenin. Src family kinases can also phosphorylate tyrosine 86, 489, and 654 in beta-Catenin. Tyr-654 phosphorylation regulates beta-Catenin binding to E-cadherin, while c-Abl phosphorylation of Tyr-489 decreases beta-Catenin binding to N-Cadherin and leads to nuclear translocation and transcriptional activation.
References
Rhee, J. et al. (2007) Nat. Cell Biol. 9(8):883.
Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225.
Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287.
Roura, S. et al. (1999) J Biol Chem. 274(51) :36734.
Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225.
Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287.
Roura, S. et al. (1999) J Biol Chem. 274(51) :36734.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,497 Da
NCBI Official Full Name
catenin beta-1
NCBI Official Synonym Full Names
catenin (cadherin-associated protein), beta 1, 88kDa
NCBI Official Symbol
CTNNB1
NCBI Official Synonym Symbols
CTNNB; MRD19; armadillo
NCBI Protein Information
catenin beta-1
UniProt Protein Name
Catenin beta-1
UniProt Gene Name
CTNNB1
UniProt Synonym Gene Names
CTNNB
UniProt Entry Name
CTNB1_HUMAN
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Product Notes
The CTNNB1 ctnnb1 (Catalog #AAA71610) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The beta-Catenin (Tyr-654)[gamma-Catenin (Tyr-644)], phospho-specific Antibody reacts with Human, Rat, Mouse, Chicken, Fish and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Catenin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CTNNB1 ctnnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Catenin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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