FCM/FACS (Flow Cytometry)
(BHLHB5 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit BHLHB5 Polyclonal Antibody | anti-BHLHE22 antibody
BHLHB5 Antibody (Center)
Gene Names
BHLHE22; Beta3; BHLHB5; CAGL85; TNRC20
Reactivity
Human (Predicted Reactivity: Chicken, Mouse)
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
BHLHB5, Antibody; BHLHB5 Antibody (Center); Class E basic helix-loop-helix protein 22; bHLHe22; Class B basic helix-loop-helix protein 5; bHLHb5; Trinucleotide repeat-containing gene 20 protein; BHLHE22; BHLHB5; TNRC20; anti-BHLHE22 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Chicken, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This BHLHB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the Central region of human BHLHB5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
236-264
Sequence Length
381
Applicable Applications for anti-BHLHE22 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Tissue Location
Brain-specific, with the highest expression in the cerebellum.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(BHLHB5 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(BHLHB5 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(BHLHB5 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BHLHB5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(BHLHB5 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BHLHB5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of BHLHB5 Antibody (Center) in HL-60 cell line lysates (35ug/lane). BHLHB5 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of BHLHB5 Antibody (Center) in HL-60 cell line lysates (35ug/lane). BHLHB5 (arrow) was detected using the purified Pab.)
Related Product Information for anti-BHLHE22 antibody
BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.
Product Categories/Family for anti-BHLHE22 antibody
References
Stevens, J.D., et al. Differentiation 76(9):1006-1022(2008)
McLellan, A.S., et al. Mech. Dev. 119 SUPPL 1, S285-S291 (2002)
McLellan, A.S., et al. Mech. Dev. 119 SUPPL 1, S285-S291 (2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36997
NCBI Official Full Name
class E basic helix-loop-helix protein 22
NCBI Official Synonym Full Names
basic helix-loop-helix family, member e22
NCBI Official Symbol
BHLHE22
NCBI Official Synonym Symbols
Beta3; BHLHB5; CAGL85; TNRC20
NCBI Protein Information
class E basic helix-loop-helix protein 22
UniProt Protein Name
Class E basic helix-loop-helix protein 22
UniProt Gene Name
BHLHE22
UniProt Synonym Gene Names
BHLHB5; TNRC20; bHLHe22; bHLHb5
UniProt Entry Name
BHE22_HUMAN
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Product Notes
The BHLHE22 bhlhe22 (Catalog #AAA286170) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 236-264. The BHLHB5 Antibody (Center) reacts with Human (Predicted Reactivity: Chicken, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's BHLHB5 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the BHLHE22 bhlhe22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BHLHB5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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