WB (Western Blot)
(Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204). HBEpC cell lysates either transiently induced (Lane 2) or noninduced with the EGF (Lane 1)(2 ug/lane).)
Rabbit Bi-Phospho-ERK1/2 (T202/Y204) Polyclonal Antibody | anti-MAPK3 antibody
Bi-Phospho-ERK1/2 (T202/Y204) Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human (Predicted Reactivity: Drosophila, Mouse, Rat)
Applications
Western Blot, ELISA, Dot Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Bi-Phospho-ERK1/2 (T202/Y204), Antibody; Bi-Phospho-ERK1/2 (T202/Y204) Antibody; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK3; ERK1; PRKM3; anti-MAPK3 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Drosophila, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ERK1/2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T202/Y204 of human ERK1/2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
357
Applicable Applications for anti-MAPK3 antibody
WB (Western Blot), ELISA, DB (Dot Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204). HBEpC cell lysates either transiently induced (Lane 2) or noninduced with the EGF (Lane 1)(2 ug/lane).)
WB (Western Blot)
(Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204). HBEpC cell lysates either transiently induced (Lane 2) or noninduced with the EGF (Lane 1)(2 ug/lane).)
WB (Western Blot)
(Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204). HAOSMC cell lysates either transiently induced (Lane 2) or noninduced with the PDGF (Lane 1)(2 ug/lane).)
WB (Western Blot)
(Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204). HAOSMC cell lysates either transiently induced (Lane 2) or noninduced with the PDGF (Lane 1)(2 ug/lane).)
DB (Dot Blot)
(Dot blot analysis of Bi-phospho-ERK1/2-T202/Y204 Antibody on nitrocellulose membrane. 50ng of Bisphospho-peptide or Non Phosphorylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
DB (Dot Blot)
(Dot blot analysis of Bi-phospho-ERK1/2-T202/Y204 Antibody on nitrocellulose membrane. 50ng of Bisphospho-peptide or Non Phosphorylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Related Product Information for anti-MAPK3 antibody
MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.
Product Categories/Family for anti-MAPK3 antibody
References
Munshi, H.G., et al., J. Biol. Chem. 279(37):39042-39050 (2004).
Mukherjee, S., et al., Infect. Immun. 72(9):5274-5282 (2004).
Sebkova, L., et al., Infect. Immun. 72(9):5019-5026 (2004).
Huang, H.M., et al., Biochem. Biophys. Res. Commun. 320(4):1247-1252 (2004).
Mizuno, S., et al., Am. J. Respir. Cell Mol. Biol. 31(2):184-192 (2004).
Mukherjee, S., et al., Infect. Immun. 72(9):5274-5282 (2004).
Sebkova, L., et al., Infect. Immun. 72(9):5019-5026 (2004).
Huang, H.M., et al., Biochem. Biophys. Res. Commun. 320(4):1247-1252 (2004).
Mizuno, S., et al., Am. J. Respir. Cell Mol. Biol. 31(2):184-192 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43136
NCBI Official Full Name
mitogen-activated protein kinase 3 isoform 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase 3
NCBI Official Symbol
MAPK3
NCBI Official Synonym Symbols
ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
NCBI Protein Information
mitogen-activated protein kinase 3
UniProt Protein Name
Mitogen-activated protein kinase 3
UniProt Gene Name
MAPK3
UniProt Synonym Gene Names
ERK1; PRKM3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK
UniProt Entry Name
MK03_HUMAN
Similar Products
Product Notes
The MAPK3 mapk3 (Catalog #AAA288905) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bi-Phospho-ERK1/2 (T202/Y204) Antibody reacts with Human (Predicted Reactivity: Drosophila, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Bi-Phospho-ERK1/2 (T202/Y204) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, DB (Dot Blot). Researchers should empirically determine the suitability of the MAPK3 mapk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bi-Phospho-ERK1/2 (T202/Y204), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.