IP (Immunoprecipitation)
(MAPK3 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate4 uL anti-MAPK3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAPK3 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size: 43 kDa)
Rabbit anti-Human ERK1/MAPK3 Polyclonal Antibody | anti-MAPK3 antibody
Anti-ERK1/MAPK3 Antibody, Rabbit Polyclonal
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
ERK1/MAPK3, Antibody; Anti-ERK1/MAPK3 Antibody, Rabbit Polyclonal; MAPK3 Antibody, Rabbit PAb, Antigen Affinity Purified; mitogen-activated protein kinase 3; Anti-ERK-1 Antibody; Anti-ERK1 Antibody; Anti-ERT2 Antibody; Anti-HS44KDAP Antibody; Anti-HUMKER1A Antibody; Anti-p44-ERK1 Antibody; Anti-p44-MAPK Antibody; Anti-P44ERK1 Antibody; Anti-P44MAPK Antibody; Anti-PRKM3 Antibody; anti-MAPK3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MAPK3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-MAPK3 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human MAPK3
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human MAPK3, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(MAPK3 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate4 uL anti-MAPK3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAPK3 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size: 43 kDa)
IP (Immunoprecipitation)
(MAPK3 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate4 uL anti-MAPK3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAPK3 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size: 43 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of MAPK3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MAPK3 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of MAPK3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MAPK3 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
WB (Western Blot)
(Anti-MAPK3 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:43 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-MAPK3 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:43 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-MAPK3 antibody
MAPK3 (Mitogen-Activated Protein Kinase 3, also known as ERK1) is a Protein Coding gene. The protein encoded by the ERK1 gene is a member of the MAP kinase family. 3 alternatively spliced human isoforms have been reported. The protein is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs that play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. ERK1 is widely expressed in the small intestine, colon, and other tissues. Diseases associated with MAPK3 include Neutrophil Migration and Pertussis.
Product Categories/Family for anti-MAPK3 antibody
References
Kolb RH, Greer PM, Cao PT, Cowan KH, Yan Y. ERK1/2 Signaling Plays an Important Role in Topoisomerase II Poison-Induced G2/M Checkpoint Activation. Tan M, ed. PLoS ONE. 2012;7(11):e50281.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,136 Da
NCBI Official Full Name
mitogen-activated protein kinase 3 isoform 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase 3
NCBI Official Symbol
MAPK3
NCBI Official Synonym Symbols
ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
NCBI Protein Information
mitogen-activated protein kinase 3; MAPK 1; MAP kinase isoform p44; insulin-stimulated MAP2 kinase; extracellular signal-related kinase 1; extracellular signal-regulated kinase 1; microtubule-associated protein 2 kinase
UniProt Protein Name
Mitogen-activated protein kinase 3
UniProt Gene Name
MAPK3
UniProt Synonym Gene Names
ERK1; PRKM3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK
UniProt Entry Name
MK03_HUMAN
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Product Notes
The MAPK3 mapk3 (Catalog #AAA254408) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ERK1/MAPK3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERK1/MAPK3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MAPK3 mapk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERK1/MAPK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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