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WB (Western Blot)
(Detection of human BIG1/ARFGEF1 by western blot. Samples: Whole cell lysate (25 ug) from RKO, LNCaP, HEK293T, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody (AAA211270 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse BIG1/ARFGEF1 Polyclonal Antibody | anti-BIG1/ARFGEF1 antibody
Rabbit anti-BIG1/ARFGEF1 Antibody, Affinity Purified
Gene Names
ARFGEF1; BIG1; P200; ARFGEP1; DKFZP434L057; D730028O18Rik
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
BIG1/ARFGEF1, Antibody; Rabbit anti-BIG1/ARFGEF1 Antibody, Affinity Purified; brefeldin A-inhibited guanine nucleotide-exchange protein 1; p200 ARF guanine nucleotide exchange factor; ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited); P200; p200 ARF-GEP1; Brefeldin A-inhibited GEP 1; ARFGEP1; ADP-ribosylation factor guanine nucleotide-exchange factor 1; BIG1; Brefeldin A-inhibited guanine nucleotide-exchange protein 1; ADP ribosylation factor guanine nucleotide exchange factor 1; anti-BIG1/ARFGEF1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-BIG1/ARFGEF1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine
Immunogen
Between 1799 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human BIG1/ARFGEF1 by western blot. Samples: Whole cell lysate (25 ug) from RKO, LNCaP, HEK293T, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody (AAA211270 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human BIG1/ARFGEF1 by western blot. Samples: Whole cell lysate (25 ug) from RKO, LNCaP, HEK293T, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody (AAA211270 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human BIG1/ARFGEF1 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, TCMK-1, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody (AAA211270 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human BIG1/ARFGEF1 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, TCMK-1, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody (AAA211270 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human BIG1/ARFGEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody (AAA211270 lot 2) used for IP at 6 ug per reaction. BIG1/ARFGEF1 was also immunoprecipitated by a previous lot of this antibody (AAA211270 lot 1) and a second antibody against a different epitope of BIG1/ARFGEF1 (Antibody 2). For blotting immunoprecipitated BIG1/ARFGEF1, AAA211270 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human BIG1/ARFGEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody (AAA211270 lot 2) used for IP at 6 ug per reaction. BIG1/ARFGEF1 was also immunoprecipitated by a previous lot of this antibody (AAA211270 lot 1) and a second antibody against a different epitope of BIG1/ARFGEF1 (Antibody 2). For blotting immunoprecipitated BIG1/ARFGEF1, AAA211270 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human BIG1/ARFGEF1 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 (Cat. No. AAA211270 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human BIG1/ARFGEF1 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 (Cat. No. AAA211270 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-BIG1/ARFGEF1 antibody
BIG1/ARFGEF1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.
Product Categories/Family for anti-BIG1/ARFGEF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
208,767 Da
NCBI Official Full Name
brefeldin A-inhibited guanine nucleotide-exchange protein 1
NCBI Official Synonym Full Names
ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
NCBI Official Symbol
ARFGEF1
NCBI Official Synonym Symbols
BIG1; P200; ARFGEP1; DKFZP434L057; D730028O18Rik
NCBI Protein Information
brefeldin A-inhibited guanine nucleotide-exchange protein 1; p200 ARF-GEP1; OTTHUMP00000227052; OTTHUMP00000227095; brefeldin A-inhibited GEP 1; p200 ARF guanine nucleotide exchange factor
UniProt Protein Name
Brefeldin A-inhibited guanine nucleotide-exchange protein 1
UniProt Gene Name
ARFGEF1
UniProt Synonym Gene Names
ARFGEP1; BIG1
UniProt Entry Name
BIG1_HUMAN
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Product Notes
The BIG1/ARFGEF1 arfgef1 (Catalog #AAA211270) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BIG1/ARFGEF1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BIG1/ARFGEF1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the BIG1/ARFGEF1 arfgef1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BIG1/ARFGEF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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