ICC (Immunocytochemistry)
(Immunocytochemistry of Bit1 in 3T3 cells with Bit1 antibody at 2 ug/mL.)
Rabbit Bit1 Polyclonal Antibody | anti-Bit1 antibody
Bit1 Antibody
Gene Names
PTRH2; PTH; BIT1; PTH2; PTH 2; CFAP37; IMNEPD; CGI-147
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column
Synonyms
Bit1, Antibody; Bit1 Antibody; Bcl-2 inhibitor of transcription 1, Peptidyl-tRNA hydrolase 2; anti-Bit1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Affinity chromatography purified via peptide column
Form/Format
Supplied in PBS containing 0.02% sodium azide.
Sequence Length
179
Applicable Applications for anti-Bit1 antibody
ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen Type
Peptide
Immunogen Description
Raised against a 15 amino acid peptide from near the amino-terminus of human Bit1.
Target Name
Bit1
Preparation and Storage
Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ICC (Immunocytochemistry)
(Immunocytochemistry of Bit1 in 3T3 cells with Bit1 antibody at 2 ug/mL.)
ICC (Immunocytochemistry)
(Immunocytochemistry of Bit1 in 3T3 cells with Bit1 antibody at 2 ug/mL.)
WB (Western Blot)
(Western blot analysis of Bit1 in A-20 cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 ug/mL.)
WB (Western Blot)
(Western blot analysis of Bit1 in A-20 cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 ug/mL.)
Related Product Information for anti-Bit1 antibody
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
Product Categories/Family for anti-Bit1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,194 Da
NCBI Official Full Name
peptidyl-tRNA hydrolase 2, mitochondrial isoform b
NCBI Official Synonym Full Names
peptidyl-tRNA hydrolase 2
NCBI Official Symbol
PTRH2
NCBI Official Synonym Symbols
PTH; BIT1; PTH2; PTH 2; CFAP37; IMNEPD; CGI-147
NCBI Protein Information
peptidyl-tRNA hydrolase 2, mitochondrial
UniProt Protein Name
Peptidyl-tRNA hydrolase 2, mitochondrial
UniProt Gene Name
PTRH2
UniProt Synonym Gene Names
BIT1; PTH2; PTH 2
UniProt Entry Name
PTH2_HUMAN
Similar Products
Product Notes
The Bit1 ptrh2 (Catalog #AAA305186) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bit1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Bit1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the Bit1 ptrh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bit1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.