Rabbit Bit1 Polyclonal Antibody | anti-PTRH2 antibody

Bit1 Antibody

Cat. Num. AAA40333

• Gene Names: PTRH2; BIT1; PTH2; CFAP37; CGI-147
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
• Purity: Bit1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Bit1, Antibody; Bit1 Antibody; Bit1; BIT1; PTH2; CGI-147; Peptidyl-tRNA hydrolase 2; mitochondrial; Bcl-2 inhibitor of transcription 1; PTH 2; peptidyl-tRNA hydrolase 2; anti-PTRH2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Bit1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 179

• Applicable Applications for anti-PTRH2 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: Bit1 antibody was raised against a 15 amino acid synthetic peptide from near the amino-terminus of human Bit1.
• Buffer: Bit1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Bit1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of Bit1 in 3T3 cells with Bit1 antibody at 20 μg/mL.)

ICC (Immunocytochemistry)

(Immunocytochemistry of Bit1 in 3T3 cells with Bit1 antibody at 2 μg/mL.)

WB (Western Blot)

(Western blot analysis of Bit1 in A-20 cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.)

Related Product Information for anti-PTRH2 antibody

Bit1 Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

NCBI and Uniprot Product Information

• NCBI GI #: 7706351
• NCBI GeneID: 51651
• NCBI Accession #: NP_057161
• NCBI GenBank Nucleotide #: NM_016077.3
• UniProt Accession #: Q9Y3E5; Q9NTE5; B3KUY4
• Molecular Weight: 19,194 Da
• NCBI Official Full Name: peptidyl-tRNA hydrolase 2, mitochondrial
• NCBI Official Synonym Full Names: peptidyl-tRNA hydrolase 2
• NCBI Official Symbol: PTRH2
• NCBI Official Synonym Symbols: BIT1; PTH2; CFAP37; CGI-147
• NCBI Protein Information: peptidyl-tRNA hydrolase 2, mitochondrial; PTH 2; bcl-2 inhibitor of transcription 1; cilia and flagella associated protein 37
• UniProt Protein Name: Peptidyl-tRNA hydrolase 2, mitochondrial
• UniProt Gene Name: PTRH2
• UniProt Synonym Gene Names: BIT1; PTH2; PTH 2
• UniProt Entry Name: PTH2_HUMAN