IHC (Immunohiostchemistry)
(Bit1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Bit1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human Bit1 Polyclonal Antibody | anti-PTRH2 antibody
Bit1 Antibody (Center)
Gene Names
PTRH2; 2; PTH; BIT1; PTH2; PTH 2; CFAP37; IMNEPD; CGI-147
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Bit1, Antibody; Bit1 Antibody (Center); Peptidyl-tRNA hydrolase 2; mitochondrial; PTH 2; Bcl-2 inhibitor of transcription 1; PTRH2; BIT1; PTH2; anti-PTRH2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Bit1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-110 amino acids from the Central region of human Bit1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
80-110
Sequence Length
179
Applicable Applications for anti-PTRH2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Bit1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Bit1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Bit1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Bit1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Bit1 Antibody (K95) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the Bit1 antibody detected the Bit1 protein (arrow).)
WB (Western Blot)
(Bit1 Antibody (K95) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the Bit1 antibody detected the Bit1 protein (arrow).)
Related Product Information for anti-PTRH2 antibody
Adhesion to extracellular matrix regulates cell survival via integrin engagement and cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. A role for Bit1 (Bcl-2 inhibitor of transcription 1) has been identified in this process. Bit1 is a mitochondrial protein released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. Bit1-AES complexes may switch off a survival-promoting gene transcription program controlled by TLE. Apoptosis of Bit1/AES transfected cells is inhibited when cells are permitted to attach to fibronectin through the alpha-beta integrin, suggesting that the contribution of the Bit1-AES pathway to anoikis is regulated by integrins.
Product Categories/Family for anti-PTRH2 antibody
References
Cell 116(5):751-762 (2004).
Biochim Biophys Acta. 1692:145-57 (2004).
Gene 2000; 249:1-16.
Biochim Biophys Acta. 1692:145-57 (2004).
Gene 2000; 249:1-16.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19194
NCBI Official Full Name
peptidyl-tRNA hydrolase 2, mitochondrial isoform b
NCBI Official Synonym Full Names
peptidyl-tRNA hydrolase 2
NCBI Official Symbol
PTRH2
NCBI Official Synonym Symbols
2; PTH; BIT1; PTH2; PTH 2; CFAP37; IMNEPD; CGI-147
NCBI Protein Information
peptidyl-tRNA hydrolase 2, mitochondrial
UniProt Protein Name
Peptidyl-tRNA hydrolase 2, mitochondrial
UniProt Gene Name
PTRH2
UniProt Synonym Gene Names
BIT1; PTH2; PTH 2
UniProt Entry Name
PTH2_HUMAN
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Product Notes
The PTRH2 ptrh2 (Catalog #AAA290018) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 80-110. The Bit1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bit1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PTRH2 ptrh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bit1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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