Rabbit anti-Human BLNK Polyclonal Antibody | anti-BLNK antibody

BLNK Antibody

Cat. Num. AAA233410

• Gene Names: BLNK; bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: BLNK, Antibody; BLNK Antibody; B-cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Cytoplasmic adapter protein; Src homology 2 domain-containing leukocyte protein of 65 kDa; SLP-65; BLNK; BASH; SLP65; anti-BLNK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 433

• Applicable Applications for anti-BLNK antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant Human B-cell linker protein (41-337AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IHC (Immunohistochemisry)

(Immunofluorescent analysis of Hela cells using AAA233410 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohiostchemistry)

(IHC image of AAA233410 diluted at 1:800 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot)

(Western BlotPositive WB detected in: Raji whole cell lysate,K562 whole cell lysate,A549 whole cell lysateAll lanes: BLNK antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 51,49,45 KDaObserved band size: 51 KDa)

Related Product Information for anti-BLNK antibody

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 166235901
• NCBI GeneID: 29760
• NCBI Accession #: NP_001107566.1
• NCBI GenBank Nucleotide #: NM_001114094.1
• UniProt Accession #: Q8WV28; O75498; O75499; Q2MD49
• Molecular Weight: 44,451 Da
• NCBI Official Full Name: B-cell linker protein isoform 2
• NCBI Official Synonym Full Names: B-cell linker
• NCBI Official Symbol: BLNK
• NCBI Official Synonym Symbols: bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• NCBI Protein Information: B-cell linker protein
• UniProt Protein Name: B-cell linker protein
• UniProt Gene Name: BLNK
• UniProt Synonym Gene Names: BASH; SLP65; SLP-65