Application Data
(Blank control: Jurkat.Primary Antibody (green line): Rabbit Anti-BRN3A antibody Dilution: 1 ug /106 cells; Isotype Control Antibody (orange line): Rabbit IgG Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 1 ug /test. The cells were fixed with 4% PFA (10 min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20 degree C.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
Rabbit BRN3A Polyclonal Antibody | anti-BRN3A antibody
BRN3A
Reactivity
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit
Applications
Immunofluorescence, ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Protein A affinity purified
Synonyms
BRN3A, Antibody; BRN3A; Brain specific homeobox/POU domain protein 3A; Brn 3.0; BRN 3A; Brn3; Homeobox/POU domain protein RDC 1; Homeobox/POU domain protein RDC1; Oct T1; OctT1; POU Domain Class 4 Transcription Factor 1; POU4F1; RDC 1; RDC1; anti-BRN3A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A affinity purified
Form/Format
0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-BRN3A antibody
IF (Immunofluorescence), ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
KLH conjugated synthetic peptide derived from human BRN3A 325-419/419
Positive control
Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye.
Subcellular location
Nucleus
Preparation and Storage
Store at -20 degree C for one year. Avoid repeated freeze/thaw cycles.
Application Data
(Blank control: Jurkat.Primary Antibody (green line): Rabbit Anti-BRN3A antibody Dilution: 1 ug /106 cells; Isotype Control Antibody (orange line): Rabbit IgG Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 1 ug /test. The cells were fixed with 4% PFA (10 min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20 degree C.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
Application Data
(Blank control: Jurkat.Primary Antibody (green line): Rabbit Anti-BRN3A antibody Dilution: 1 ug /106 cells; Isotype Control Antibody (orange line): Rabbit IgG Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 1 ug /test. The cells were fixed with 4% PFA (10 min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20 degree C.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
Application Data
(U-937 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRN3A Antibody at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).)
Application Data
(U-937 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRN3A Antibody at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).)
WB (Western Blot)
(Sample: Cerebrum (Mouse) Lysate at 40 ug.Spinal cord (Mouse) Lysate at 40 ug.Primary: Anti-BRN3A at 1/500 dilution.Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution.Predicted band size: 43 kD.Observed band size: 33 kD.)
WB (Western Blot)
(Sample: Cerebrum (Mouse) Lysate at 40 ug.Spinal cord (Mouse) Lysate at 40 ug.Primary: Anti-BRN3A at 1/500 dilution.Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution.Predicted band size: 43 kD.Observed band size: 33 kD.)
Related Product Information for anti-BRN3A antibody
Multifunctional transcription factor with different regions mediating its different effects. Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that activation of some of these genes requires its N-terminal domain,maybe through a neuronal-specific cofactor. Ativates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain). Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
43 KDa
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Product Notes
The BRN3A (Catalog #AAA268973) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRN3A reacts with Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's BRN3A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the BRN3A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRN3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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