FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of CACO-2 cells using anti-BRSK2 antibody (AAA126424).Overlay histogram showing CACO-2 cells stained with AAA126424 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRSK2 Antibody (AAA126424, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit BRSK2 Polyclonal Antibody | anti-BRSK2 antibody
Anti-BRSK2 Antibody Picoband
Gene Names
BRSK2; SAD1; SADA; STK29; PEN11B; C11orf7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
BRSK2, Antibody; Anti-BRSK2 Antibody Picoband; anti-BRSK2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-BRSK2 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human BRSK2 recombinant protein (Position: M250-P736).
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of CACO-2 cells using anti-BRSK2 antibody (AAA126424).Overlay histogram showing CACO-2 cells stained with AAA126424 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRSK2 Antibody (AAA126424, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of CACO-2 cells using anti-BRSK2 antibody (AAA126424).Overlay histogram showing CACO-2 cells stained with AAA126424 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRSK2 Antibody (AAA126424, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of BRSK2 using anti-BRSK2 antibody (AAA126424).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human CACO-2 whole cell lysates,Lane 2: human SH-SY5Y whole cell lysates,Lane 3: rat testis tissue lysates,Lane 4: mouse brain tissue lysates,Lane 5: mouse testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRSK2 antigen affinity purified polyclonal antibody (#AAA126424) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BRSK2 at approximately 90 kDa. The expected band size for BRSK2 is at 90 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of BRSK2 using anti-BRSK2 antibody (AAA126424).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human CACO-2 whole cell lysates,Lane 2: human SH-SY5Y whole cell lysates,Lane 3: rat testis tissue lysates,Lane 4: mouse brain tissue lysates,Lane 5: mouse testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRSK2 antigen affinity purified polyclonal antibody (#AAA126424) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BRSK2 at approximately 90 kDa. The expected band size for BRSK2 is at 90 kDa.)
Related Product Information for anti-BRSK2 antibody
BR serine/threonine-protein kinase 2 is an enzyme that in humans is encoded by the BRSK2 gene. BRSK2 was initially identified through a computer screen of the human genome and shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK2 is expressed in the brain and to a lesser extent in the testes. BRSK2 is a member of the AMP-activated protein kinase subfamily and can be activated by the tumor suppressor kinase LKB1. More recently, it has been shown that both BRSK2 and the related protein BRSK1 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. BRSK2 has also been shown to be an autoantigen in paraneoplastic limbic encephalitis. At least four isoforms of BRSK2 are known to exist.
Product Categories/Family for anti-BRSK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
68,740 Da
NCBI Official Full Name
Serine/threonine-protein kinase BRSK2
NCBI Official Synonym Full Names
BR serine/threonine kinase 2
NCBI Official Symbol
BRSK2
NCBI Official Synonym Symbols
SAD1; SADA; STK29; PEN11B; C11orf7
NCBI Protein Information
serine/threonine-protein kinase BRSK2; protein kinase SAD1B; brain-selective kinase 2; serine/threonine-protein kinase 29; BR serine/threonine-protein kinase 2; serine/threonine-protein kinase SAD-A; brain-specific serine/threonine-protein kinase 2
UniProt Protein Name
Serine/threonine-protein kinase BRSK2
UniProt Gene Name
BRSK2
UniProt Synonym Gene Names
C11orf7; PEN11B; SADA; STK29; BR serine/threonine-protein kinase 2
UniProt Entry Name
BRSK2_HUMAN
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Product Notes
The BRSK2 brsk2 (Catalog #AAA126424) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BRSK2 Antibody Picoband reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BRSK2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the BRSK2 brsk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRSK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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