Rabbit BSCL2 Polyclonal Antibody | anti-BSCL2 antibody

BSCL2 Antibody (N-term)

Cat. Num. AAA286757

• Gene Names: BSCL2; HMN5; PELD; SPG17; GNG3LG
• Reactivity: Human, Mouse (Predicted Reactivity: Bovine)
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: BSCL2, Antibody; BSCL2 Antibody (N-term); Seipin; Bernardinelli-Seip congenital lipodystrophy type 2 protein; BSCL2; anti-BSCL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This BSCL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-107 amino acids from the N-terminal region of human BSCL2.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 78-107
• Sequence Length: 462

• Applicable Applications for anti-BSCL2 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Plays a crucial role in the formation of lipid droplets (LDs) which are storage organelles at the center of lipid and energy homeostasis (PubMed:19278620, PubMed:21533227, PubMed:30293840, PubMed:31708432). In association with LDAF1, defines the sites of LD formation in the ER (PubMed:31708432). Also required for growth and maturation of small nascent LDs into larger mature LDs (PubMed:27564575). Mediates the formation and/or stabilization of endoplasmic reticulum-lipid droplets (ER-LD) contacts, facilitating protein and lipid delivery from the ER into growing LDs (PubMed:27879284, PubMed:31178403). Regulates the maturation of ZFYVE1- positive nascent LDs and the function of the RAB18-ZFYVE1 complex in mediating the formation of ER-LD contacts (PubMed:30970241). Binds anionic phospholipids including phosphatidic acid (PubMed:30293840). Plays an important role in the differentiation and development of adipocytes (By similarity).
• Cellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Lipid droplet Note=Localizes at endoplasmic reticulum-lipid droplets (ER-LD) contact sites.
• Tissue Location: Expressed in motor neurons in the spinal cord and cortical neurons in the frontal lobe (at protein level). Highly expressed in brain, testis and adipose tissue
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(All lanes : Anti-BSCL2 Antibody (N-term) at 1:2000 dilution Lane 1: Human testis lysate Lane 2: Mouse testis lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

IHC (Immunohiostchemistry)

(BSCL2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BSCL2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(BSCL2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the BSCL2 antibody detected the BSCL2 protein (arrow).)

Related Product Information for anti-BSCL2 antibody

This gene encodes protein seipin, which is located in the endoplasmic reticulum and may be important for lipid droplet morphology. Mutations in this gene have been associated with congenital generalized lipodystrophy type 2 or Berardinelli-Seip syndrome, a rare autosomal recessive disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-BSCL2 antibody

Cardiovascular; Metabolism; Neuroscience

References

Rakocevic-Stojanovic, V., et al. J. Neurol. Sci. 296 (1-2), 107-109 (2010) :
Luigetti, M., et al. Muscle Nerve 42(3):448-451(2010)
Nishiyama, A., et al. Pediatr Int 51(6):775-779(2009)
Wu, Y.R., et al. J. Neurol. Neurosurg. Psychiatr. 80(10):1180-1181(2009)
Chen, W., et al. Endocrinology 150(10):4552-4561(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 171906569
• NCBI GeneID: 26580
• NCBI Accession #: NP_001116427.1; NP_001124174.2; NP_116056.3
• NCBI GenBank Nucleotide #: NM_001122955.3
• UniProt Accession #: Q96G97; Q567S1; Q96SV1; Q9BSQ0; G3XAE4
• NCBI Official Full Name: seipin isoform 1
• NCBI Official Synonym Full Names: Berardinelli-Seip congenital lipodystrophy 2 (seipin)
• NCBI Official Symbol: BSCL2
• NCBI Official Synonym Symbols: HMN5; PELD; SPG17; GNG3LG
• NCBI Protein Information: seipin
• UniProt Protein Name: Seipin
• UniProt Gene Name: BSCL2
• UniProt Entry Name: BSCL2_HUMAN