WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using BTRC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Rabbit BTRC Polyclonal Antibody | anti-BTRC antibody
BTRC Polyclonal Antibody
Gene Names
BTRC; FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Synonyms
BTRC, Antibody; BTRC Polyclonal Antibody; BETA-TRCP; FBW1A; FBXW1; FBXW1A; FWD1; MGC4643; bTrCP; bTrCP1; betaTrCP; anti-BTRC antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
605
Applicable Applications for anti-BTRC antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human BTRC
Calculated Molecular Weight
69kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using BTRC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using BTRC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Related Product Information for anti-BTRC antibody
beta-transducin repeat-containing protein (beta-TrCP or FBW1A) is an F-box family protein characterized by the presence of the protein-protein mediating F-box domain first described in cyclin F. F-box proteins act as substrate adaptors that target proteins containing a specific phosphorylated sequence element, referred to as a phosphodegron, to the SCF E3 ubiquitin ligase complex for ubiquitination (1,2). beta-TrCP targets many important proteins with diverse functions, such as p53, H-Ras, Smad4, IkappaBalpha, beta-catenin, and the cell cycle checkpoint protein claspin, for ubiquitin-mediated degradation (3-5). Research studies have shown that inhibition of beta-TrCP expression has a demonstrated benefit in the treatment of prostate cancer (6).
Product Categories/Family for anti-BTRC antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
605
NCBI Official Full Name
F-box/WD repeat-containing protein 1A
NCBI Official Synonym Full Names
beta-transducin repeat containing E3 ubiquitin protein ligase
NCBI Official Symbol
BTRC
NCBI Official Synonym Symbols
FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP
NCBI Protein Information
F-box/WD repeat-containing protein 1A; beta-TrCP1; E3RSIkappaB; F-box and WD-repeat protein 1B; epididymis tissue protein Li 2a; pIkappaBalpha-E3 receptor subunit; F-box and WD repeats protein beta-TrCP
UniProt Protein Name
F-box/WD repeat-containing protein 1A
UniProt Gene Name
BTRC
UniProt Synonym Gene Names
BTRCP; FBW1A; FBXW1A
UniProt Entry Name
FBW1A_HUMAN
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Product Notes
The BTRC btrc (Catalog #AAA37056) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BTRC Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BTRC can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the BTRC btrc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BTRC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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