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IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using C9orf72 Rabbit pAb (AAA28290) at dilution of 1:100 (40x lens).)
Rabbit C9orf72 Polyclonal Antibody | anti-C9orf72 antibody
C9orf72 Polyclonal Antibody
Gene Names
C9orf72; ALSFTD; DENND9; FTDALS; DENNL72; FTDALS1
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
C9orf72; Polyclonal Antibody; C9orf72 Polyclonal Antibody; ALSFTD; DENNL72; FTDALS; FTDALS1; protein C9orf72; anti-C9orf72 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
481
Applicable Applications for anti-C9orf72 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:100
IF 1:50 - 1:200
IHC: 1:50-1:100
IF 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human C9orf72 (NP_060795.1).
Species Reactivity/Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using C9orf72 Rabbit pAb (AAA28290) at dilution of 1:100 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using C9orf72 Rabbit pAb (AAA28290) at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human esophageal cancer using C9orf72 Rabbit pAb (AAA28290) at dilution of 1:100 (40x lens))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human esophageal cancer using C9orf72 Rabbit pAb (AAA28290) at dilution of 1:100 (40x lens))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using C9orf72Rabbit pAb (AAA28290) at dilution of 1:100 (40xlens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using C9orf72Rabbit pAb (AAA28290) at dilution of 1:100 (40xlens).)
WB (Western Blot)
(Western blot analysis of extracts of Rat brain, using C9orf72 antibody (AAA28290) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
WB (Western Blot)
(Western blot analysis of extracts of Rat brain, using C9orf72 antibody (AAA28290) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using C9orf72 antibody (AAA28290) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020). Exposure time: 90s)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using C9orf72 antibody (AAA28290) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020). Exposure time: 90s)
WB (Western Blot)
(Western blot analysis of extracts of mouse kidney, using C9orf72 antibody (AAA28290) at 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of extracts of mouse kidney, using C9orf72 antibody (AAA28290) at 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-C9orf72 antibody
The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093). Alternative splicing results in multiple transcript variants encoding different isoforms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed MW: 50KDa
Calculated MW: 24kDa/54kDa
Calculated MW: 24kDa/54kDa
NCBI Official Full Name
guanine nucleotide exchange C9orf72 isoform a
NCBI Official Synonym Full Names
C9orf72-SMCR8 complex subunit
NCBI Official Symbol
C9orf72
NCBI Official Synonym Symbols
ALSFTD; DENND9; FTDALS; DENNL72; FTDALS1
NCBI Protein Information
guanine nucleotide exchange C9orf72
UniProt Protein Name
Protein C9orf72
UniProt Gene Name
C9orf72
UniProt Entry Name
CI072_HUMAN
Similar Products
Product Notes
The C9orf72 c9orf72 (Catalog #AAA28290) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's C9orf72 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:100 IF 1:50 - 1:200. Researchers should empirically determine the suitability of the C9orf72 c9orf72 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C9orf72, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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