IP (Immunoprecipitation)
(mCPB1 was immunoprecipitated using:Lane A:0.5 mg Mouse Spleen Whole Cell Lysate4 uL anti-mCPB1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mCPB1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size :40 kDa)
Rabbit anti-Mouse Carboxypeptidase B1 Polyclonal Antibody
Anti-Carboxypeptidase B1 Antibody, Rabbit Polyclonal
Reactivity
Mouse
Applications
Immunohistochemistry, Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Carboxypeptidase B1, Antibody; Anti-Carboxypeptidase B1 Antibody, Rabbit Polyclonal; carboxypeptidase B1 (tissue); Anti-0910001A18Rik Antibody; Anti-1810063F02Rik Antibody; Anti-2210008M23Rik Antibody; Anti-AI504870 Antibody; anti-Carboxypeptidase B1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse Carboxypeptidase B1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-Carboxypeptidase B1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Mouse Carboxypeptidase B1 Protein
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Carboxypeptidase B1 (rM Carboxypeptidase B1). Carboxypeptidase B1 specific IgG was purified by Mouse Carboxypeptidase B1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(mCPB1 was immunoprecipitated using:Lane A:0.5 mg Mouse Spleen Whole Cell Lysate4 uL anti-mCPB1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mCPB1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size :40 kDa)
IP (Immunoprecipitation)
(mCPB1 was immunoprecipitated using:Lane A:0.5 mg Mouse Spleen Whole Cell Lysate4 uL anti-mCPB1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mCPB1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size :40 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of human Carboxypeptidase B1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human Carboxypeptidase B1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-CPB1 rabbit polyclonal antibody at 1:1000 dilutionLane A: Mouse spleen tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:47 kDaObserved band size:47 kDa)
WB (Western Blot)
(Anti-CPB1 rabbit polyclonal antibody at 1:1000 dilutionLane A: Mouse spleen tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:47 kDaObserved band size:47 kDa)
Related Product Information for anti-Carboxypeptidase B1 antibody
Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point. The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
References
Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581.
Pezzilli, R. et al., 1994, Digestion. 55: 73-77.
Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.
Pezzilli, R. et al., 1994, Digestion. 55: 73-77.
Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.
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Product Notes
The Carboxypeptidase B1 (Catalog #AAA259129) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Carboxypeptidase B1 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Carboxypeptidase B1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Carboxypeptidase B1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carboxypeptidase B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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