WB (Western Blot)
(Detection of human CAV1 by western blot. Samples: Whole cell lysate (50 ug) from GaMG, MCF-7, A-549, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAV1 antibody (lot AAA213490 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human CAV1 Polyclonal Antibody | anti-CAV1 antibody
Rabbit anti-CAV1 Antibody, Affinity Purified
Gene Names
CAV1; CGL3; BSCL3; VIP21; MSTP085
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CAV1, Antibody; Rabbit anti-CAV1 Antibody, Affinity Purified; VIP21; PPH3; MSTP085; LCCNS; CGL3; cell growth-inhibiting protein 32; caveolin-1; BSCL3; caveolin 1, caveolae protein, 22kDa; Caveolin-1; caveolin 1; anti-CAV1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CAV1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat, Sheep, Bovine, Horse, Rabbit, Pig, Cat, Orangutan, Gorilla, Chimpanzee
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CAV1 by western blot. Samples: Whole cell lysate (50 ug) from GaMG, MCF-7, A-549, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAV1 antibody (lot AAA213490 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human CAV1 by western blot. Samples: Whole cell lysate (50 ug) from GaMG, MCF-7, A-549, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAV1 antibody (lot AAA213490 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CAV1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from GaMG cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAV1 antibody (AAA213490 lot 1) used for IP at 1 ug per reaction. CAV1 was also immunoprecipitated by a second antibody against a different epitope of CAV1 (Antibody 2). For blotting immunoprecipitated CAV1, AAA213490 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CAV1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from GaMG cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAV1 antibody (AAA213490 lot 1) used for IP at 1 ug per reaction. CAV1 was also immunoprecipitated by a second antibody against a different epitope of CAV1 (Antibody 2). For blotting immunoprecipitated CAV1, AAA213490 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-CAV1 antibody
CAV1 acts as a scaffolding protein within caveolar membranes and forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. CAV1 also mediates the recruitment of CAVIN proteins to the caveolae and interacts directly with G-protein alpha subunits and can functionally regulate their activity. CAV1 is also thought to be involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation, as its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q03135].
Product Categories/Family for anti-CAV1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,472 Da
NCBI Official Full Name
caveolin-1 isoform alpha
NCBI Official Synonym Full Names
caveolin 1, caveolae protein, 22kDa
NCBI Official Symbol
CAV1
NCBI Official Synonym Symbols
CGL3; BSCL3; VIP21; MSTP085
NCBI Protein Information
caveolin-1; cell growth-inhibiting protein 32
UniProt Protein Name
Caveolin-1
UniProt Gene Name
CAV1
UniProt Synonym Gene Names
CAV
UniProt Entry Name
CAV1_HUMAN
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Product Notes
The CAV1 cav1 (Catalog #AAA213490) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CAV1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CAV1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CAV1 cav1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAV1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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