Goat CAV1 / Caveolin 1 Polyclonal Antibody | anti-CAV1 antibody

Anti-CAV1 / Caveolin 1 Antibody (N-Terminus) IHC-plus

Cat. Num. AAA162202

• Gene Names: CAV1; CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085
• Reactivity: Mouse, Dog, Human; Predicted Reactivity: Monkey, Canine (at least 90% immunogen sequence identity)
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Immunoaffinity purified
• Synonyms: CAV1 / Caveolin 1, Antibody; Anti-CAV1 / Caveolin 1 Antibody (N-Terminus) IHC-plus; Goat Polyclonal (IgG) to Human CAV1 / Caveolin 1; Human CAV1 / Caveolin 1; Caveolin-1; CGL3; CAV; MSTP085; VIP21; BSCL3; anti-CAV1 antibody

• Host: Goat
• Reactivity: Mouse, Dog, Human; Predicted Reactivity: Monkey, Canine (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects a band of approximately 25 kDa by Western blot in the following cell lysate: hCat, MDCK and 3T3.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, 20% glycerol, 0.05% sodium azide.
• Concentration: 2 mg/ml (varies by lot)
• Sequence Length: 178

• Applicable Applications for anti-CAV1 antibody: WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
• Immunogen: CAV1 / Caveolin 1 antibody was raised against purified recombinant peptide derived from within residues 100 aa to the N-terminus of human CAV1 produced in E Coli.
• Immunogen Type: Recombinant protein
• Immunogen Species: CAV1 / Caveolin 1 antibody was raised against Human
• Antigen Modification: N-Terminus
• Target: CAV1 / Caveolin 1
• Epitope: N-Terminus
• Predicted Reativity: Monkey, Dog
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only:; Tissue Preparation:; Formalin fixation and embedding in paraffin wax; Tissue Sectioning:; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-drying oven for 45 minutes at 60°C.; Deparaffinization:; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; Rehydration:; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; Antigen retrieval; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) - 1 minute @ RT; Immunostaining:; (Do not allow tissues to dry at any time during the staining procedure); Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropiate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST - 1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST - 1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; Dehydrate:; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red DAB); Wash slides in 2 changes of 80% alcohol- 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol-1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Recommended Storage: Store at 4 degree C short term.; Store at -20 degree C long term.; Avoid freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 15451856
• NCBI GeneID: 857
• NCBI Accession #: NP_001744.2
• NCBI GenBank Nucleotide #: NM_001753.4
• UniProt Accession #: Q03135; Q9UGP1; Q9UNG1; Q9UQH6
• Molecular Weight: 17,023 Da
• NCBI Official Full Name: caveolin-1 isoform alpha
• NCBI Official Synonym Full Names: caveolin 1
• NCBI Official Symbol: CAV1
• NCBI Official Synonym Symbols: CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085
• NCBI Protein Information: caveolin-1
• UniProt Protein Name: Caveolin-1
• UniProt Gene Name: CAV1
• UniProt Synonym Gene Names: CAV
• UniProt Entry Name: CAV1_HUMAN