IP (Immunoprecipitation)
(Detection of human Cbl-b by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cbl-b antibody AAA212068 used for IP at 3 ug/mg lysate. Cbl-b was also immunoprecipitated by rabbit anti-Cbl-b antibody which recognizes a downstream epitope. For blotting immunoprecipitated Cbl-b, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human Cbl-b Polyclonal Antibody | anti-Cbl-b antibody
Rabbit anti-Cbl-b Antibody, Affinity Purified
Gene Names
CBLB; Cbl-b; RNF56; Nbla00127
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
Cbl-b, Antibody; Rabbit anti-Cbl-b Antibody, Affinity Purified; RING-type E3 ubiquitin transferase CBL-B; signal transduction protein CBL-B; RNF56; RING finger protein 56; Nbla00127; Cbl-b; Cbl proto-oncogene, E3 ubiquitin protein ligase B; Cbl proto-oncogene B, E3 ubiquitin protein ligase; casitas B-lineage lymphoma proto-oncogene b; Cas-Br-M (murine) ecotropic retroviral transforming sequence b; SH3-binding protein CBL-B; E3 ubiquitin-protein ligase CBL-B; Cbl proto-oncogene B; anti-Cbl-b antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Cbl-b antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Mouse, Rat
Immunogen
Between 475 and 525
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human Cbl-b by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cbl-b antibody AAA212068 used for IP at 3 ug/mg lysate. Cbl-b was also immunoprecipitated by rabbit anti-Cbl-b antibody which recognizes a downstream epitope. For blotting immunoprecipitated Cbl-b, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Cbl-b by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cbl-b antibody AAA212068 used for IP at 3 ug/mg lysate. Cbl-b was also immunoprecipitated by rabbit anti-Cbl-b antibody which recognizes a downstream epitope. For blotting immunoprecipitated Cbl-b, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human Cbl-b by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Cbl-b (Cat. No. AAA212068 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Cbl-b by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Cbl-b (Cat. No. AAA212068 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-Cbl-b antibody
Cbl-b is an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Cbl-b negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13191].
Product Categories/Family for anti-Cbl-b antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86,582 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase CBL-B
NCBI Official Synonym Full Names
Cbl proto-oncogene B, E3 ubiquitin protein ligase
NCBI Official Symbol
CBLB
NCBI Official Synonym Symbols
Cbl-b; RNF56; Nbla00127
NCBI Protein Information
E3 ubiquitin-protein ligase CBL-B
UniProt Protein Name
E3 ubiquitin-protein ligase CBL-B
UniProt Gene Name
CBLB
UniProt Synonym Gene Names
RNF56
UniProt Entry Name
CBLB_HUMAN
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Product Notes
The Cbl-b cblb (Catalog #AAA212068) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Cbl-b Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cbl-b can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the Cbl-b cblb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cbl-b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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