IF (Immunofluorescence)
(Immunofluorescence of CCL2 in human spleen tissue with CCL2 antibody at 20 μg/ml.)
Rabbit CCL2 Polyclonal Antibody | anti-CCL2 antibody
CCL2 Antibody
Gene Names
CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
CCL2 Antibody is affinity chromatography purified via peptide column.
Synonyms
CCL2, Antibody; CCL2 Antibody; CCL2 antibody: HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30; C-C motif chemokine 2; HC11; chemokine (C-C motif) ligand 2; anti-CCL2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
CCL2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; CCL2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
99
Applicable Applications for anti-CCL2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-paraffin (IHC-P), Immunofluorescence (IF)
Application Notes
CCL2 antibody can be used for detection of CCL2 by Western blot at 1 - 2 ug/mL.
Optimal dilutions for each application to be determined by the researcher.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples
and Immunofluorescence in human samples. All other applications and species not yet tested.
Optimal dilutions for each application to be determined by the researcher.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples
and Immunofluorescence in human samples. All other applications and species not yet tested.
Conjugate
Unconjugated
Immunogen
Rabbit polyclonal CCL2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human CCL2.
The immunogen is located within the last 50 amino acids of CCL2.
The immunogen is located within the last 50 amino acids of CCL2.
Homology
Predicted species reactivity based on immunogen sequence: Pig: (81%), Horse: (81%), Bovine: (75%), Rabbit: (75%), Guinea pig: (90%)
Preparation and Storage
CCL2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
IF (Immunofluorescence)
(Immunofluorescence of CCL2 in human spleen tissue with CCL2 antibody at 20 μg/ml.)
IF (Immunofluorescence)
(Immunofluorescence of CCL2 in human spleen tissue with CCL2 antibody at 20 μg/ml.)
IHC (Immunohistochemistry)
(Immunohistochemistry of CCL2 in human spleen tissue with CCL2 antibody at 5 μg/ml.)
IHC (Immunohistochemistry)
(Immunohistochemistry of CCL2 in human spleen tissue with CCL2 antibody at 5 μg/ml.)
WB (Western Blot)
(Western blot analysis of CCL2 in K562 cell lysate with CCL2 antibody at 1 μg/mL.)
WB (Western Blot)
(Western blot analysis of CCL2 in K562 cell lysate with CCL2 antibody at 1 μg/mL.)
Related Product Information for anti-CCL2 antibody
CCL2 Antibody: CCL2, also known as monocyte chemotactic protein 1 (MCP1), belongs to the intercrine beta (chemokine CC) family. It is produced by a variety of cell types and is a potent chemoattractant for monocytes, memory T lymphocytes, and natural killer (NK) cells. It is upregulated during infection and inflammation. CCL2 is a potent basophil activator but does not affect eosinophils, whereas the related protein MCP2 stimulates both eosinophils and basophils. MCP3 has been shown to have the broadest range of influence. CCL2 has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 11kDa
Observed: 14kDa
Observed: 14kDa
NCBI Official Full Name
C-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 2
NCBI Official Symbol
CCL2
NCBI Official Synonym Symbols
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
NCBI Protein Information
C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
UniProt Protein Name
C-C motif chemokine 2
UniProt Gene Name
CCL2
UniProt Synonym Gene Names
MCP1; SCYA2; MCAF; MCP-1
UniProt Entry Name
CCL2_HUMAN
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Product Notes
The CCL2 ccl2 (Catalog #AAA10976) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CCL2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CCL2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-paraffin (IHC-P), Immunofluorescence (IF). CCL2 antibody can be used for detection of CCL2 by Western blot at 1 - 2 ug/mL. Optimal dilutions for each application to be determined by the researcher. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the CCL2 ccl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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