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FCM/FACS (Flow Cytometry)
(Flow cytometric of HeLa cells using CCL2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human CCL2 / MCP1 Polyclonal Antibody | anti-CCL2 antibody
Anti-CCL2 / MCP1 Antibody (aa62-89) IHC-plus
Gene Names
CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Ammonium sulfate precipitation
Synonyms
CCL2 / MCP1, Antibody; Anti-CCL2 / MCP1 Antibody (aa62-89) IHC-plus; Rabbit Polyclonal to Human CCL2 / MCP1; Human CCL2 / MCP1; C-C motif chemokine 2; Chemokine (C-C motif) ligand 2; GDCF-2; HC11; HSMCR30; SCYA2; Small-inducible cytokine A2; Monocyte secretory protein JE; MCAF; MCP-1; Monocyte chemotactic protein 1; SMC-CF; anti-CCL2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human CCL2 / MCP-1
Purity/Purification
Ammonium sulfate precipitation
Form/Format
PBS, 0.09% sodium azide
Sequence Length
99
Applicable Applications for anti-CCL2 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
Immunogen
CCL2 / MCP1 antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human CCL2.
Immunogen Description
KLH-conjugated synthetic peptide from C-terminal region of human CCL2.
Immunogen Type
Synthetic peptide
Antigen Modification
aa62-89
Target Species
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.
For long term storage store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric of HeLa cells using CCL2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric of HeLa cells using CCL2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot of CCL2 Antibody in HeLa cell line lysates (35 ug/lane). CCL2 (arrow) was detected using the purified antibody.)
WB (Western Blot)
(Western blot of CCL2 Antibody in HeLa cell line lysates (35 ug/lane). CCL2 (arrow) was detected using the purified antibody.)
IF (Immunofluorescence)
(Immunofluorescence of CCL2 Antibody with HeLa cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)
IF (Immunofluorescence)
(Immunofluorescence of CCL2 Antibody with HeLa cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)
IF (Immunofluorescence)
(Confocal immunofluorescence of CCL2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescence of CCL2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohistochemistry)
(Anti-CCL2 / MCP1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.)
IHC (Immunohistochemistry)
(Anti-CCL2 / MCP1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.)
Related Product Information for anti-CCL2 antibody
CCL2 Antibody, C-C motif chemokine 2 Antibody, Chemokine (C-C motif) ligand 2 Antibody, GDCF-2 Antibody, HC11 Antibody, HSMCR30 Antibody, MCP1 Antibody, SCYA2 Antibody, Small-inducible cytokine A2 Antibody, Monocyte secretory protein JE Antibody, MCAF Antibody, MCP-1 Antibody, Monocyte chemotactic protein 1 Antibody, SMC-CF Antibody Description: CCL2 / MCP1 is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
C-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 2
NCBI Official Symbol
CCL2
NCBI Official Synonym Symbols
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
NCBI Protein Information
C-C motif chemokine 2
UniProt Protein Name
C-C motif chemokine 2
UniProt Gene Name
CCL2
UniProt Synonym Gene Names
MCP1; SCYA2; MCAF; MCP-1
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Product Notes
The CCL2 ccl2 (Catalog #AAA162177) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CCL2 / MCP1 Antibody (aa62-89) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCL2 / MCP1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CCL2 ccl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCL2 / MCP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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