WB (Western Blot)
(Detection of human and mouse CCT8 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CCT8 antibody AAA212170 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. CCT8 was also immunoprecipitated by rabbit anti-CCT8 antibodies A303-448 and which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
Rabbit anti-Human, Mouse CCT8 Polyclonal Antibody | anti-CCT8 antibody
Rabbit anti-CCT8 Antibody, Affinity Purified
Gene Names
CCT8; Cctq; PRED71; D21S246; C21orf112
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CCT8, Antibody; Rabbit anti-CCT8 Antibody, Affinity Purified; CCT-theta; T-complex protein 1 subunit theta; renal carcinoma antigen NY-REN-15; TCP-1-theta; PRED71; chaperonin containing T-complex polypeptide 1 subunit 8; Cctq; C21orf112; chaperonin containing TCP1, subunit 8 (theta); D21S246; chaperonin containing TCP1 subunit 8; anti-CCT8 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-CCT8 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Chicken, Bovine, Orangutan
Immunogen
Between 400 and 450
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse CCT8 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CCT8 antibody AAA212170 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. CCT8 was also immunoprecipitated by rabbit anti-CCT8 antibodies A303-448 and which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human and mouse CCT8 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CCT8 antibody AAA212170 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. CCT8 was also immunoprecipitated by rabbit anti-CCT8 antibodies A303-448 and which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
IHC (Immunohiostchemistry)
(Detection of human CCT8 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CCT8 (Cat. No. AAA212170 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
IHC (Immunohiostchemistry)
(Detection of human CCT8 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CCT8 (Cat. No. AAA212170 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of mouse CCT8 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CCT8 (Cat. No. AAA212170 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse CCT8 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CCT8 (Cat. No. AAA212170 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-CCT8 antibody
CCT8 is also known as T-complex protein 1 subunit theta (TCP1-theta) and is a molecular chaperone that assists in the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis and regulating the transport of vesicles to the cilia. TCP1-theta is known to play a role, in vitro, in the folding of actin and tubulin [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P50990].
Product Categories/Family for anti-CCT8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,621 Da
NCBI Official Full Name
T-complex protein 1 subunit theta
NCBI Official Synonym Full Names
chaperonin containing TCP1, subunit 8 (theta)
NCBI Official Symbol
CCT8
NCBI Official Synonym Symbols
Cctq; PRED71; D21S246; C21orf112
NCBI Protein Information
T-complex protein 1 subunit theta; CCT-theta; TCP-1-theta; renal carcinoma antigen NY-REN-15; T-complex protein 1, theta subunit
UniProt Protein Name
T-complex protein 1 subunit theta
UniProt Gene Name
CCT8
UniProt Synonym Gene Names
C21orf112; CCTQ; KIAA0002; TCP-1-theta
UniProt Entry Name
TCPQ_HUMAN
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Product Notes
The CCT8 cct8 (Catalog #AAA212170) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CCT8 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CCT8 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CCT8 cct8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCT8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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