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IP (Immunoprecipitation)
(ANPEP was immunoprecipitated using:Lane A:0.5 mg THP1 Whole Cell Lysate2 uL anti-ANPEP rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ANPEP rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size: 110 kDa)
Rabbit anti-Human CD13 Polyclonal Antibody | anti-ANPEP antibody
Anti-CD13 Antibody, Rabbit Polyclonal
Gene Names
ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
CD13, Antibody; Anti-CD13 Antibody, Rabbit Polyclonal; CD13/ANPEP Antibody, Rabbit PAb, Antigen Affinity Purified; alanyl (membrane) aminopeptidase; Anti-APN Antibody; Anti-CD13 Antibody; Anti-GP150 Antibody; Anti-LAP1 Antibody; Anti-P150 Antibody; Anti-PEPN Antibody; anti-ANPEP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ANPEP/CD13
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ANPEP antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human ANPEP protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human ANPEP (rh ANPEP). ANPEP specific IgG was purified by human ANPEP affinity chromatography
.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(ANPEP was immunoprecipitated using:Lane A:0.5 mg THP1 Whole Cell Lysate2 uL anti-ANPEP rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ANPEP rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size: 110 kDa)
IP (Immunoprecipitation)
(ANPEP was immunoprecipitated using:Lane A:0.5 mg THP1 Whole Cell Lysate2 uL anti-ANPEP rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ANPEP rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size: 110 kDa)
WB (Western Blot)
(Anti-ANPEP rabbit polyclonal antibody at 1:500 dilutionLane A: U937 Whole Cell LysateLane B: THP-1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:110 kDaObserved band size:130 kDa)
WB (Western Blot)
(Anti-ANPEP rabbit polyclonal antibody at 1:500 dilutionLane A: U937 Whole Cell LysateLane B: THP-1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:110 kDaObserved band size:130 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of human ANPEP in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human ANPEP in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human ANPEP in human renal carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human ANPEP in human renal carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-ANPEP antibody
Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.
References
Pasqualini R, et al. (2000) Aminopeptidase N is a receptor for tumor-homing peptides and a target for inhibiting angiogenesis.Cancer Res. 60(3): 722-7.Mabjeesh SJ, et al. (2005) Aminopeptidase N gene expression and abundance in caprine mammary gland is influenced by circulating plasma peptide. J Dairy Sci. 88(6): 2055-64.Dunkel B, et al. (2009) Neutrophil and platelet activation in equine recurrent airway obstruction is associated with increased neutrophil CD13 expression, but not platelet CD41/61 and CD62P or neutrophil-platelet aggregate formation. Vet Immunol Immunopathol. 131(1-2): 25-32.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109,540 Da
NCBI Official Full Name
aminopeptidase N
NCBI Official Synonym Full Names
alanyl (membrane) aminopeptidase
NCBI Official Symbol
ANPEP
NCBI Official Synonym Symbols
APN; CD13; LAP1; P150; PEPN; GP150
NCBI Protein Information
aminopeptidase N; AP-M; AP-N; hAPN; aminopeptidase M; alanyl aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13
UniProt Protein Name
Aminopeptidase N
UniProt Gene Name
ANPEP
UniProt Synonym Gene Names
APN; CD13; PEPN; AP-N; hAPN; AP-M
UniProt Entry Name
AMPN_HUMAN
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Product Notes
The ANPEP anpep (Catalog #AAA254266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD13 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD13 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ANPEP anpep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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