WB (Western Blot)
(Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using CD18/ITGB2 (Phospho-Thr758) Antibody. The lane on the right is blocked with the phospho peptide.)
Rabbit CD18/ITGB2 Polyclonal Antibody | anti-CD18/ITGB2 antibody
CD18/ITGB2 (Phospho-Thr758) Antibody
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
CD18/ITGB2, Antibody; CD18/ITGB2 (Phospho-Thr758) Antibody; CD18; cell surface adhesion glycoproteins LFA- 1/CR3/p150,95 beta; complement receptor C3 beta; integrin beta-2; ITB2; anti-CD18/ITGB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
CD18/ITGB2 (Phospho-Thr758) Antibody detects endogenous levels of CD18/ITGB2 only when phosphorylated at Thr758.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
769
Applicable Applications for anti-CD18/ITGB2 antibody
ELISA, IF (Immunofluorescence), WB (Western Blot)
Target Modification
Phospho
Modification Sites
H:T758; M:T760; R:T757
Immunogen
The antiserum was produced against synthesized peptide derived from human CD18/ITGB2 around the phosphorylation site of Thr758.
Immunogen Range
720-769
Preparation and Storage
Stable at -20 degree C for at least 1 year.
WB (Western Blot)
(Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using CD18/ITGB2 (Phospho-Thr758) Antibody. The lane on the right is blocked with the phospho peptide.)
WB (Western Blot)
(Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using CD18/ITGB2 (Phospho-Thr758) Antibody. The lane on the right is blocked with the phospho peptide.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HepG2 cells, using CD18/ITGB2 (Phospho-Thr758) Antibody. The picture on the right is blocked with the phospho peptide.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HepG2 cells, using CD18/ITGB2 (Phospho-Thr758) Antibody. The picture on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84 kDa
NCBI Official Full Name
integrin beta-2 isoform 1
NCBI Official Synonym Full Names
integrin subunit beta 2
NCBI Official Symbol
ITGB2
NCBI Official Synonym Symbols
LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
NCBI Protein Information
integrin beta-2
UniProt Protein Name
Integrin beta-2
UniProt Gene Name
ITGB2
UniProt Synonym Gene Names
CD18; MFI7
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Product Notes
The CD18/ITGB2 itgb2 (Catalog #AAA316326) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD18/ITGB2 (Phospho-Thr758) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD18/ITGB2 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the CD18/ITGB2 itgb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD18/ITGB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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