anti-Human, Mouse CD20 Polyclonal Antibody | anti-CD20 antibody

Anti-CD20 Antibody IHC-plus

Cat. Num. AAA21271

• Gene Names: MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Caprylic acid and ammonium sulfate precipitation
• Synonyms: CD20, Antibody; Anti-CD20 Antibody IHC-plus; Rabbit Polyclonal (IgG) to Mouse CD20; Mouse CD20; MS4A1; B-lymphocyte antigen CD20; B1; Bp35; CD20; CD20 antigen; CD20 receptor; LEU-16; CVID5; anti-CD20 antibody

• Host: CD20 antibody was produced in Rabbit.
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Mouse CD20
• Purity/Purification: Caprylic acid and ammonium sulfate precipitation
• Form/Format: PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
• Concentration: 3.12 mg/ml (varies by lot)
• Sequence Length: 297

• Applicable Applications for anti-CD20 antibody: Immunohistochemistry (IHC) Paraffin (IHC-P), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC-P (5 ug/ml); ; Optimal dilution to be determined by the researcher.
• Immunogen: CD20 antibody was raised against recombinant mouse B-lymphocyte antigen CD20 protein.
• Immunogen Species: CD20 antibody was raised against Mouse.
• Immunogen Type: Recombinant protein.
• Target: CD20
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

IHC (Immunohistochemistry-Paraffin)

(Mouse Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE))

NCBI and Uniprot Product Information

• NCBI GI #: 23110989
• NCBI GeneID: 931
• NCBI Accession #: NP_690605.1
• NCBI GenBank Nucleotide #: NM_152866.2
• UniProt Accession #: P11836; P08984; Q13963; A6NMS4; B4DT24
• NCBI Official Full Name: B-lymphocyte antigen CD20
• NCBI Official Synonym Full Names: membrane spanning 4-domains A1
• NCBI Official Symbol: MS4A1
• NCBI Official Synonym Symbols: B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• NCBI Protein Information: B-lymphocyte antigen CD20
• UniProt Protein Name: B-lymphocyte antigen CD20
• UniProt Gene Name: MS4A1
• UniProt Synonym Gene Names: CD20
• UniProt Entry Name: CD20_HUMAN