IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CD294 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Rabbit anti-Human, Rat CD294 Polyclonal Antibody | anti-CD294 antibody
Anti-CD294 Antibody
Gene Names
PTGDR2; DP2; DL1R; CD294; CRTH2; GPR44
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
CD294, Antibody; Anti-CD294 Antibody; anti-CD294 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of CD294 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH7.3, 30% glycerol, and 0.01% sodium azide.
Applicable Applications for anti-CD294 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD294. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CD294 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CD294 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Western blot analysis of CD294 expression in HEK293T (A), HuT78 (B), rat spleen (C) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 43 kD))
WB (Western Blot)
(Western blot analysis of CD294 expression in HEK293T (A), HuT78 (B), rat spleen (C) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 43 kD))
Related Product Information for anti-CD294 antibody
Rabbit polyclonal antibody to CD294
Product Categories/Family for anti-CD294 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 43, 55 kDa
Obderved: 60kDa
Obderved: 60kDa
NCBI Official Full Name
prostaglandin D2 receptor 2
NCBI Official Synonym Full Names
prostaglandin D2 receptor 2
NCBI Official Symbol
PTGDR2
NCBI Official Synonym Symbols
DP2; DL1R; CD294; CRTH2; GPR44
NCBI Protein Information
prostaglandin D2 receptor 2; G protein-coupled receptor 44; G-protein coupled receptor 44; putative G-protein coupled receptor 44; chemoattractant receptor-homologous molecule expressed on TH2 cells; chemoattractant receptor homologous molecule expressed
UniProt Protein Name
Prostaglandin D2 receptor 2
UniProt Gene Name
PTGDR2
UniProt Synonym Gene Names
CRTH2; DL1R; GPR44
UniProt Entry Name
PD2R2_HUMAN
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Product Notes
The CD294 ptgdr2 (Catalog #AAA265106) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD294 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD294 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD294 ptgdr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD294, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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