anti-Human CD294 Antibody | anti-CD294 antibody

Anti-Hu CD294 Purified

Cat. Num. AAA129272

• Gene Names: PTGDR2; DP2; DL1R; CD294; CRTH2; GPR44
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD294; N/A; Anti-Hu CD294 Purified; prostaglandin D2 receptor 2; DP2; PTGDR2; DL1R; CRTH2; GPR44; anti-CD294 antibody

• Reactivity: Human
• Isotype: Rat IgG2a
• Clone Number: BM16
• Specificity: The rat monoclonal antibody BM16 recognizes an extracellular epitope of CD294/CRTH2 (prostaglandin D2 receptor 2), a G-protein-coupled seven-transmembrane protein expressed on Th2 cells, peripheral blood basophils and eosinophils.
• Form/Format: Purified; Purified by protein-G affinity chromatography.; Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD294 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: Human CD294 transfected rat cell line TART/B19-12.10
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD294 positive lymphocytes (red-filled) from CD294 negative lymphocytes in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD294/CRTH2 (BM16) purified antibody (concentration in sample 5,0 ?g/ml, GAR APC))

FCM/FACS (Flow Cytometry)

(Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD294/CRTH2 (BM16) purified antibody (concentration in sample 5,0 ?g/ml, GAR APC) and anti-human CD4 (MEM-241) Pacific Blue™ antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)

Related Product Information for anti-CD294 antibody

CD294 (prostaglandin D2 receptor 2) is a G-protein-coupled receptor that is preferentially expressed in CD4+ effector T helper 2 (Th2) cells, but also on eosinophils and basophils. It mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. Single nucleotide polymorphisms in the 3' UTR of CD294 gene have been associated with asthma susceptibility. Outside the immune system CD294 is expressed e.g. in gut, heart, and brain. The intracellular C terminal tail contains sites for phosphorylation by protein kinase C.

References

*De Fanis U, Mori F, Kurnat RJ, Lee WK, Bova M, Adkinson NF, Casolaro V: GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells. GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells. Blood. 2007 May 15;109(10):4343-50. Epub 2007 Jan 18., URL: https://pubmed.ncbi.nlm.nih.gov/17234745/,*Mjösberg J, Bernink J, Golebski K, Karrich JJ, Peters CP, Blom B, te Velde AA, Fokkens WJ, van Drunen CM, Spits H: The transcription factor GATA3 is essential for the function of human type 2 innate lymphoid cells. Immunity. 2012 Oct 19;37(4):649-59., URL: https://pubmed.ncbi.nlm.nih.gov/23063330/,*Bellinghausen I, Reuter S, Martin H, Maxeiner J, Luxemburger U, Türeci Ö, Grabbe S, Taube C, Saloga J: Enhanced production of CCL18 by tolerogenic dendritic cells is associated with inhibition of allergic airway reactivity. J Allergy Clin Immunol. 2012 Dec;130(6):1384-93., URL: http://www.ncbi.nlm.nih.gov/pubmed/23102918,*Nagata K, Tanaka K, Ogawa K, Kemmotsu K, Imai T, Yoshie O, Abe H, Tada K, Nakamura M, Sugamura K, Takano S: Selective expression of a novel surface molecule by human Th2 cells in vivo. J Immunol. 1999 Feb 1;162(3):1278-86., URL: http://www.ncbi.nlm.nih.gov/pubmed/9973380,

NCBI and Uniprot Product Information

• NCBI GI #: 153791424
• NCBI GeneID: 11251
• NCBI Accession #: NP_004769
• NCBI GenBank Nucleotide #: NM_004778.2
• UniProt Accession #: Q9Y5Y4; O94765; Q4QRI6
• Molecular Weight: Predicted: 43, 55 kDa; Obderved: 60kDa
• NCBI Official Full Name: prostaglandin D2 receptor 2
• NCBI Official Synonym Full Names: prostaglandin D2 receptor 2
• NCBI Official Symbol: PTGDR2
• NCBI Official Synonym Symbols: DP2; DL1R; CD294; CRTH2; GPR44
• NCBI Protein Information: prostaglandin D2 receptor 2; G protein-coupled receptor 44; G-protein coupled receptor 44; putative G-protein coupled receptor 44; chemoattractant receptor-homologous molecule expressed on TH2 cells; chemoattractant receptor homologous molecule expressed
• UniProt Protein Name: Prostaglandin D2 receptor 2
• UniProt Gene Name: PTGDR2
• UniProt Synonym Gene Names: CRTH2; DL1R; GPR44
• UniProt Entry Name: PD2R2_HUMAN