WB (Western Blot)
(CD320 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the CD320 antibody detected the CD320 protein (arrow).)
Rabbit anti-Human CD320 Polyclonal Antibody | anti-CD320 antibody
CD320 Antibody (C-term)
Gene Names
CD320; 8D6; 8D6A; TCBLR
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CD320, Antibody; CD320 Antibody (C-term); CD320 antigen; 8D6 antigen; FDC-signaling molecule 8D6; FDC-SM-8D6; Transcobalamin receptor; TCblR; CD320; 8D6A; anti-CD320 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CD320 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-182 amino acids from the C-terminal region of human CD320.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
153-182
Sequence Length
240
Applicable Applications for anti-CD320 antibody
ELISA, WB (Western Blot)
Function
Receptor for transcobalamin saturated with cobalamin (TCbl) (PubMed: ). Plays an important role in cobalamin uptake (PubMed: , PubMed:20524213). Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal center B cells (PubMed: ). Functions as costimulator to promote B cell responses to antigenic stimuli; promotes B cell differentiation and proliferation (PubMed: , PubMed: ). Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC) (PubMed: ). CD320 augments the proliferation of PC precursors generated by IL-10 (PubMed: ).
Cellular Location
Cell membrane; Single-pass type I membrane protein
Tissue Location
Detected in the germinal center (GC) of lymphoid follicles (at protein level) (PubMed:11418631). Expressed abundantly on follicular dendritic cells (FDCs) (PubMed:10727470)
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(CD320 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the CD320 antibody detected the CD320 protein (arrow).)
WB (Western Blot)
(CD320 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the CD320 antibody detected the CD320 protein (arrow).)
Related Product Information for anti-CD320 antibody
The CD320 antigen gene encodes the transcobalamin receptor (TCBLR). Cellular uptake of cobalamin (vitamin B12) is mediated by receptors expressed on the cell surface. Transcobalamin II (TCN2;MIM 613441), a plasma protein secreted by endothelial cells, binds the cobalamin absorbed in the distal ileum and carries 10 to 30% of total circulating cobalamin. CD320 encodes a transcobalamin receptor that binds TCN2-cobalamin at the plasma membrane and internalizes the complex by endocytosis (Quadros et al., 2009 [PubMed 18779389]).
Product Categories/Family for anti-CD320 antibody
References
Jiang, W., et al. Gene 466 (1-2), 49-55 (2010) :
Pangilinan, F., et al. J. Med. Genet. 47(10):677-685(2010)
Quadros, E.V., et al. Hum. Mutat. 31(8):924-929(2010)
Quadros, E.V., et al. Blood 113(1):186-192(2009)
Cho, W., et al. BMB Rep 41(12):863-867(2008)
Pangilinan, F., et al. J. Med. Genet. 47(10):677-685(2010)
Quadros, E.V., et al. Hum. Mutat. 31(8):924-929(2010)
Quadros, E.V., et al. Blood 113(1):186-192(2009)
Cho, W., et al. BMB Rep 41(12):863-867(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28991
NCBI Official Full Name
CD320 antigen isoform 2
NCBI Official Synonym Full Names
CD320 molecule
NCBI Official Symbol
CD320
NCBI Official Synonym Symbols
8D6; 8D6A; TCBLR
NCBI Protein Information
CD320 antigen
UniProt Protein Name
CD320 antigen
UniProt Gene Name
CD320
UniProt Synonym Gene Names
8D6A; FDC-SM-8D6; TCblR
UniProt Entry Name
CD320_HUMAN
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Product Notes
The CD320 cd320 (Catalog #AAA289592) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 153-182. The CD320 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD320 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD320 cd320 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD320, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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