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IHC (Immunohistchemistry)
(CD40 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit CD40 Polyclonal Antibody | anti-CD40 antibody
CD40 Rabbit anti-Human Polyclonal Antibody
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Reactivity
Mouse, Rat, Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide affinity chromatography.
Synonyms
CD40; Polyclonal Antibody; CD40 Rabbit anti-Human Polyclonal Antibody; IHC-plus CD40 Antibody; B-cell surface antigen CD40; B cell-associated molecule; B cell surface antigen CD40; CD40 antigen; CD40 type II isoform; p50; Bp50; CD40L receptor; CDW40; TNFRSF5; anti-CD40 antibody
Host
Rabbit
Reactivity
Mouse, Rat, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
CD40 Antibody detects endogenous levels of total CD40.
Purity/Purification
Peptide affinity chromatography.
Form/Format
PBS, pH7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD40 antibody
Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB)
Application Notes
IF: 1:100-1:500
IHC-P: 10ug/ml
Peptide-ELISA: 1:20000-1:40000
WB: 1:500-1:2000
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking.
IHC-P: 10ug/ml
Peptide-ELISA: 1:20000-1:40000
WB: 1:500-1:2000
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking.
Target
Human CD40
Immunogen
Synthesized peptide derived from human CD40
Conjugation
Unconjugated
Family
TNF Receptor
Preparation and Storage
Upon receipt, store at -20 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistchemistry)
(CD40 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistchemistry)
(CD40 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CD40 Antibody-Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CD40 Antibody-Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CD40 Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CD40 Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(CD40 Antibody-Western blot analysis of CD40 expression in COS7 cells. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(CD40 Antibody-Western blot analysis of CD40 expression in COS7 cells. The lane on the left is treated with the antigen-specific peptide.)
ICC (Immunocytochemistry)
(CD40 Antibody-Staining COLO205 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25 degree C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
ICC (Immunocytochemistry)
(CD40 Antibody-Staining COLO205 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25 degree C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
ICC (Immunocytochemistry)
(CD40 Antibody)
ICC (Immunocytochemistry)
(CD40 Antibody)
Related Product Information for anti-CD40 antibody
CD40 antibody is an unconjugated rabbit polyclonal antibody to CD40 from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,619 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 5 isoform 1
NCBI Official Synonym Full Names
CD40 molecule, TNF receptor superfamily member 5
NCBI Official Symbol
CD40
NCBI Official Synonym Symbols
p50; Bp50; CDW40; TNFRSF5
NCBI Protein Information
tumor necrosis factor receptor superfamily member 5; CD40L receptor; CD40 type II isoform; B cell-associated molecule; B cell surface antigen CD40; B-cell surface antigen CD40; CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor recept
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 5
UniProt Gene Name
CD40
UniProt Synonym Gene Names
TNFRSF5
UniProt Entry Name
TNR5_HUMAN
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Product Notes
The CD40 cd40 (Catalog #AAA21366) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD40 Rabbit anti-Human Polyclonal Antibody reacts with Mouse, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD40 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB). IF: 1:100-1:500 IHC-P: 10ug/ml Peptide-ELISA: 1:20000-1:40000 WB: 1:500-1:2000 For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking. Researchers should empirically determine the suitability of the CD40 cd40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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