WB (Western Blot)
(Western blot analysis of CDC20 (arrow) using rabbit polyclonal CDC20 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC20 gene.)
Rabbit CDC20 Polyclonal Antibody | anti-CDC20 antibody
CDC20 Antibody (N-term)
Gene Names
CDC20; CDC20A; p55CDC; bA276H19.3
Reactivity
Human (Predicted Reactivity: Pig)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CDC20, Antibody; CDC20 Antibody (N-term); Cell division cycle protein 20 homolog; p55CDC; CDC20; anti-CDC20 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Pig)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CDC20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-84 amino acids from the N-terminal region of human CDC20.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Positions
57-84
Sequence Length
499
Applicable Applications for anti-CDC20 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of CDC20 (arrow) using rabbit polyclonal CDC20 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC20 gene.)
WB (Western Blot)
(Western blot analysis of CDC20 (arrow) using rabbit polyclonal CDC20 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC20 gene.)
FCM/FACS (Flow Cytometry)
(CDC20 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CDC20 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of CDC20 Antibody (N-term) in Hela cell line lysates (35ug/lane). CDC20 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of CDC20 Antibody (N-term) in Hela cell line lysates (35ug/lane). CDC20 (arrow) was detected using the purified Pab.)
Related Product Information for anti-CDC20 antibody
CDC20 is an activating regulatory factor for the APC/C (anaphase promoting complex/cyclosome). It activates the ubiquitination activity of the APC/C. CDC20 confers a strict destruction-box (D-box) dependence on APC. Levels of CDC20, as well as its binding to APC, peak in mitosis and decrease drastically at early G1.
Product Categories/Family for anti-CDC20 antibody
References
Ge S., et.al., Cell Cycle 8:167-171(2009).
Gauci S., et.al., Anal. Chem. 81:4493-4501(2009).
Gauci S., et.al., Anal. Chem. 81:4493-4501(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54723
NCBI Official Full Name
cell division cycle protein 20 homolog
NCBI Official Synonym Full Names
cell division cycle 20
NCBI Official Symbol
CDC20
NCBI Official Synonym Symbols
CDC20A; p55CDC; bA276H19.3
NCBI Protein Information
cell division cycle protein 20 homolog
UniProt Protein Name
Cell division cycle protein 20 homolog
UniProt Gene Name
CDC20
UniProt Entry Name
CDC20_HUMAN
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Product Notes
The CDC20 cdc20 (Catalog #AAA288010) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 57-84. The CDC20 Antibody (N-term) reacts with Human (Predicted Reactivity: Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's CDC20 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CDC20 cdc20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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