WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: HeLa; B: HeLa nucleus) using a 10 % SDS PAGE gel and CDCA1 antibody at a dilution of 1:5000)
Rabbit CDCA1 Polyclonal Antibody | anti-CDCA1 antibody
CDCA1 antibody
Gene Names
NUF2; CDCA1; CT106; NUF2R
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
CDCA1 antibody was purified by antigen-affinity chromatography
Synonyms
CDCA1, Antibody; CDCA1 antibody; Polyclonal CDCA1; Anti-CDCA1; CDCA 1; hNuf2R; cell division cycle associated 1; NUF2; kinetochore protein Nuf2; cell division cycle-associated protein 1; CDCA1; hsNuf2; NUF2R; cancer/testis antigen 106; CT106; CDCA-1; anti-CDCA1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
CDCA1 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.35 mg/ml (varies by lot)
Sequence Length
464
Applicable Applications for anti-CDCA1 antibody
WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence)
Biological Significance
This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: HeLa; B: HeLa nucleus) using a 10 % SDS PAGE gel and CDCA1 antibody at a dilution of 1:5000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: HeLa; B: HeLa nucleus) using a 10 % SDS PAGE gel and CDCA1 antibody at a dilution of 1:5000)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using CDCA1 antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using CDCA1 antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Related Product Information for anti-CDCA1 antibody
Affinity purified Rabbit polyclonal CDCA1 antibody
Product Categories/Family for anti-CDCA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
54,304 Da
NCBI Official Full Name
cell division cycle associated 1, isoform CRA_a
NCBI Official Synonym Full Names
NUF2, NDC80 kinetochore complex component
NCBI Official Symbol
NUF2
NCBI Official Synonym Symbols
CDCA1; CT106; NUF2R
NCBI Protein Information
kinetochore protein Nuf2
UniProt Protein Name
Kinetochore protein Nuf2
UniProt Gene Name
NUF2
UniProt Synonym Gene Names
CDCA1; NUF2R; hNuf2; hNuf2R; hsNuf2
UniProt Entry Name
NUF2_HUMAN
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Product Notes
The CDCA1 nuf2 (Catalog #AAA107872) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CDCA1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the CDCA1 nuf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDCA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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