Rabbit Chk1 Polyclonal Antibody | anti-CHEK1 antibody

Anti-Chk1 Antibody, Rabbit Polyclonal

Cat. Num. AAA254589

• Gene Names: CHEK1; CHK1
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Chk1, Antibody; Anti-Chk1 Antibody, Rabbit Polyclonal; Chk1 Antibody, Rabbit PAb, Antigen Affinity Purified; checkpoint kinase 1; Anti-CHK1 Antibody; anti-CHEK1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Chk1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CHEK1 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the center region of the Human Chk1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Chk1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence staining of CHEK1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CHEK1 polyclonal antibody (dilution ratio 1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.)

WB (Western Blot)

(Anti-CHEK1 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:54 kDa)

Related Product Information for anti-CHEK1 antibody

CHK1/CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1/CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1/CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1/CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1/CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.

Product Categories/Family for anti-CHEK1 antibody

Cancer Drug Targets Tumor Suppressors; p53 Pathway

References

Chen P, et al. (2000) The 1.7 a crystal structure of human cell cycle checkpoint kinase CHK1/CHEK1: Implications for CHK1/CHEK1 regulation. Cell. 100 (6): 681-92.
Sanchez Y, et al. (1997) Conservation of the CHK1/CHEK1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science. 277 (5331): 1497-501.
Flaggs G, et al. (1998) Atm-dependent interactions of a mammalian CHK1/CHEK1 homolog with meiotic chromosomes. Curr Biol. 7 (12): 977-86.
Chini CC, et al. (2005) Claspin, a regulator of CHK1/CHEK1 in DNA replication stress pathway. DNA Repair. 3 (8-9): 1033-7.

NCBI and Uniprot Product Information

• NCBI GI #: 166295194
• NCBI GeneID: 1111
• NCBI Accession #: NP_001107593.1
• NCBI GenBank Nucleotide #: NM_001114121.2
• UniProt Accession #: O14757; A8K934; B4DDD0; B4DSK3; B5BTY6; F5H7S4; H2BI51
• Molecular Weight: 54,434 Da
• NCBI Official Full Name: serine/threonine-protein kinase Chk1 isoform 1
• NCBI Official Synonym Full Names: checkpoint kinase 1
• NCBI Official Symbol: CHEK1
• NCBI Official Synonym Symbols: CHK1
• NCBI Protein Information: serine/threonine-protein kinase Chk1; Chk1-S; checkpoint kinase-1; CHK1 checkpoint homolog; cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1
• UniProt Protein Name: Serine/threonine-protein kinase Chk1
• UniProt Gene Name: CHEK1
• UniProt Synonym Gene Names: CHK1
• UniProt Entry Name: CHK1_HUMAN