WB (Western Blot)
(Western blot analysis of lysates from human brain and human placenta tissue (from left to right), using CHMP4A Antibody (C-term). AAA289616 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Rabbit anti-Human CHMP4A Polyclonal Antibody | anti-CHMP4A antibody
CHMP4A Antibody (C-term)
Gene Names
CHMP4A; SNF7; CHMP4; SHAX2; CHMP4B; SNF7-1; VPS32A; HSPC134; VPS32-1; C14orf123
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CHMP4A, Antibody; CHMP4A Antibody (C-term); Charged multivesicular body protein 4a; Chromatin-modifying protein 4a; CHMP4a; SNF7 homolog associated with Alix-2; SNF7-1; hSnf-1; Vacuolar protein sorting-associated protein 32-1; Vps32-1; hVps32-1; CHMP4A; C14orf123; SHAX2; anti-CHMP4A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CHMP4A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 205-239 amino acids from the C-terminal region of human CHMP4A.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
265
Applicable Applications for anti-CHMP4A antibody
ELISA, WB (Western Blot)
Function
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4A filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed: ).
Cellular location
Cytoplasmic vesicle membrane. Late endosome membrane; Peripheral membrane protein Note=Membrane-associated. Localizes to large vesicle-like structures. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
Tissue Location
Widely expressed. Expressed at higher level in heart, kidney, liver and skeletal muscle.Also expressed in brain, placenta, lung and pancreas
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from human brain and human placenta tissue (from left to right), using CHMP4A Antibody (C-term). AAA289616 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from human brain and human placenta tissue (from left to right), using CHMP4A Antibody (C-term). AAA289616 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Related Product Information for anti-CHMP4A antibody
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4A filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release.
Product Categories/Family for anti-CHMP4A antibody
References
Katoh K.,et al.J. Biol. Chem. 278:39104-39113(2003).
Peck J.W.,et al.Biochem. J. 377:693-700(2004).
Li Y.,et al.Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
Li W.B.,et al.Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
Peck J.W.,et al.Biochem. J. 377:693-700(2004).
Li Y.,et al.Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
Li W.B.,et al.Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25098
NCBI Official Full Name
charged multivesicular body protein 4a
NCBI Official Synonym Full Names
charged multivesicular body protein 4A
NCBI Official Symbol
CHMP4A
NCBI Official Synonym Symbols
SNF7; CHMP4; SHAX2; CHMP4B; SNF7-1; VPS32A; HSPC134; VPS32-1; C14orf123
NCBI Protein Information
charged multivesicular body protein 4a
UniProt Protein Name
Charged multivesicular body protein 4a
UniProt Gene Name
CHMP4A
UniProt Synonym Gene Names
C14orf123; SHAX2; CHMP4a; hSnf-1; Vps32-1; hVps32-1
UniProt Entry Name
CHM4A_HUMAN
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Product Notes
The CHMP4A chmp4a (Catalog #AAA289616) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHMP4A Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHMP4A can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CHMP4A chmp4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHMP4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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