IF (Immunofluorescence)
(Figure 2. IF analysis of Claudin 5/CLDN5 using anti- Claudin 5/CLDN5 antibody (AAA125688).Claudin 5/CLDN5 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti- Claudin 5/CLDN5 Antibody (AAA125688) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Rabbit anti-Human Claudin 5/CLDN5 Polyclonal Antibody | anti-CLDN5 antibody
Anti-Claudin 5/CLDN5 Antibody
Gene Names
CLDN5; AWAL; BEC1; TMVCF; CPETRL1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
Claudin 5/CLDN5, Antibody; Anti-Claudin 5/CLDN5 Antibody; CLDN5; AWAL; TMVCF; Claudin-5; Transmembrane protein deleted in VCFS; TMDVCF; claudin 5; anti-CLDN5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for Claudin 5/CLDN5 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Applicable Applications for anti-CLDN5 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry)
Immunogen
A synthetic peptide corresponding to a sequence of human Claudin 5/CLDN5 (KYSAPRRPTATGDYDKKNYV).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IF (Immunofluorescence)
(Figure 2. IF analysis of Claudin 5/CLDN5 using anti- Claudin 5/CLDN5 antibody (AAA125688).Claudin 5/CLDN5 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti- Claudin 5/CLDN5 Antibody (AAA125688) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IF (Immunofluorescence)
(Figure 2. IF analysis of Claudin 5/CLDN5 using anti- Claudin 5/CLDN5 antibody (AAA125688).Claudin 5/CLDN5 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti- Claudin 5/CLDN5 Antibody (AAA125688) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IHC (Immunohistochemistry)
(Figure 1. IHC analysis of Claudin 5/CLDN5 using anti-Claudin 5/CLDN5 antibody (AAA125688).Claudin 5/CLDN5 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Claudin 5/CLDN5 Antibody (AAA125688) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 1. IHC analysis of Claudin 5/CLDN5 using anti-Claudin 5/CLDN5 antibody (AAA125688).Claudin 5/CLDN5 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Claudin 5/CLDN5 Antibody (AAA125688) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Related Product Information for anti-CLDN5 antibody
Claudin-5 is a protein that in humans is encoded by the CLDN5 gene. This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
References
1. Sirotkin, H., Morrow, B., Saint-Jore, B., Puech, A., Das Gupta, R., Patanjali, S. R., Skoultchi, A., Weissman, S. M., Kucherlapati, R. Identification, characterization, and precise mapping of a human gene encoding a novel membrane-spanning protein from the 22q11 region deleted in velo-cardio-facial syndrome.
2. Peacock RE, Keen TJ, Inglehearn CF (Mar 1998). "Analysis of a human gene homologous to rat ventral prostate. 1 protein". Genomics 46 (3): 443-9.
3. "Entrez Gene: CLDN5 claudin 5 (transmembrane protein deleted in velocardiofacial syndrome)"
2. Peacock RE, Keen TJ, Inglehearn CF (Mar 1998). "Analysis of a human gene homologous to rat ventral prostate. 1 protein". Genomics 46 (3): 443-9.
3. "Entrez Gene: CLDN5 claudin 5 (transmembrane protein deleted in velocardiofacial syndrome)"
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,147 Da
NCBI Official Full Name
claudin-5
NCBI Official Synonym Full Names
claudin 5
NCBI Official Symbol
CLDN5
NCBI Official Synonym Symbols
AWAL; BEC1; TMVCF; CPETRL1
NCBI Protein Information
claudin-5; TMDVCF; transmembrane protein deleted in VCFS; transmembrane protein deleted in velocardiofacial syndrome
UniProt Protein Name
Claudin-5
UniProt Gene Name
CLDN5
UniProt Synonym Gene Names
AWAL; TMVCF; TMDVCF
UniProt Entry Name
CLD5_HUMAN
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Product Notes
The CLDN5 cldn5 (Catalog #AAA125688) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Claudin 5/CLDN5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Claudin 5/CLDN5 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CLDN5 cldn5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Claudin 5/CLDN5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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