WB (Western Blot)
(Detection of human and mouse CNOT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CNOT1 antibody (AAA213417 lot 2) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse CNOT1 Polyclonal Antibody | anti-CNOT1 antibody
Rabbit anti-CNOT1 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CNOT1, Antibody; Rabbit anti-CNOT1 Antibody, Affinity Purified; CDC39; NOT1H; NOT1 (negative regulator of transcription 1, yeast) homolog; NOT1; hNOT1; CCR4-NOT transcription complex subunit 1; CCR4-associated factor 1; adrenal gland protein AD-005; AD-005; negative regulator of transcription subunit 1 homolog; anti-CNOT1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CNOT1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Zebrafish, X. tropicalis
Immunogen
Between 1400 and 1450
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse CNOT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CNOT1 antibody (AAA213417 lot 2) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse CNOT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CNOT1 antibody (AAA213417 lot 2) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CNOT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-CNOT1 antibody AAA213417 (lot AAA213417-2) used for IP at 100 ul per reaction. CNOT1 was also immunoprecipitated by a previous lot of this antibody (lot AAA213417). For blotting immunoprecipitated CNOT1, AAA213417 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CNOT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-CNOT1 antibody AAA213417 (lot AAA213417-2) used for IP at 100 ul per reaction. CNOT1 was also immunoprecipitated by a previous lot of this antibody (lot AAA213417). For blotting immunoprecipitated CNOT1, AAA213417 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-CNOT1 antibody
Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of emryonic stem (ES) cell identity. [taken from NCBI Entrez Gene (Gene ID: 23019)]. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/A5YKK6].
Product Categories/Family for anti-CNOT1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The CNOT1 cnot1 (Catalog #AAA213417) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CNOT1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CNOT1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CNOT1 cnot1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CNOT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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