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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of CEM cells using CNOT8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit CNOT8 Polyclonal Antibody | anti-CNOT8 antibody
CNOT8 Antibody (C-term)
Gene Names
CNOT8; CAF1; POP2; CALIF; Caf1b; hCAF1
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CNOT8, Antibody; CNOT8 Antibody (C-term); CCR4-NOT transcription complex subunit 8; CAF1-like protein; CALIFp; CAF2; CCR4-associated factor 8; Caf1b; CNOT8; CALIF; POP2; anti-CNOT8 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CNOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-255 amino acids from the C-terminal region of human CNOT8.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.406 (varies by lot)
Sequence Positions
227-255
Sequence Length
238
Applicable Applications for anti-CNOT8 antibody
WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of CEM cells using CNOT8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of CEM cells using CNOT8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with CNOT8 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CNOT8 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with CNOT8 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CNOT8 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.)
WB (Western Blot)
(CNOT8 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the CNOT8 antibody detected the CNOT8 protein (arrow).)
WB (Western Blot)
(CNOT8 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the CNOT8 antibody detected the CNOT8 protein (arrow).)
Related Product Information for anti-CNOT8 antibody
Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex.
Product Categories/Family for anti-CNOT8 antibody
References
Morel,A.P., J. Cell. Sci. 116 (PT 14), 2929-2936 (2003)
Prevot,D., J. Biol. Chem. 276 (13), 9640-9648 (2001)
Prevot,D., J. Biol. Chem. 276 (13), 9640-9648 (2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33540
NCBI Official Full Name
CCR4-NOT transcription complex subunit 8 isoform 1
NCBI Official Synonym Full Names
CCR4-NOT transcription complex, subunit 8
NCBI Official Symbol
CNOT8
NCBI Official Synonym Symbols
CAF1; POP2; CALIF; Caf1b; hCAF1
NCBI Protein Information
CCR4-NOT transcription complex subunit 8
UniProt Protein Name
CCR4-NOT transcription complex subunit 8
UniProt Gene Name
CNOT8
UniProt Synonym Gene Names
CALIF; POP2; CALIFp
UniProt Entry Name
CNOT8_HUMAN
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Product Notes
The CNOT8 cnot8 (Catalog #AAA283896) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 227-255. The CNOT8 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's CNOT8 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the CNOT8 cnot8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CNOT8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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