Rabbit anti-Human, Mouse Cofilin 2 Polyclonal Antibody | anti-CFL2 antibody

Anti-Cofilin 2 Antibody, Rabbit Polyclonal

Cat. Num. AAA254520

• Gene Names: CFL2; NEM7
• Reactivity: Human, Mouse
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Cofilin 2, Antibody; Anti-Cofilin 2 Antibody, Rabbit Polyclonal; cofilin 2/CFL2 Antibody, Rabbit PAb, Antigen Affinity Purified; cofilin 2 (muscle); Anti-NEM7 Antibody; anti-CFL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Cofilin 2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CFL2 antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human cofilin 2/CFL2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human cofilin 2/CFL2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(CFL2 was immunoprecipitated using:Lane A:0.5 mg Raji Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate4 uL anti-CFL2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CFL2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size: 18 kDa)

IF (Immunofluorescence)

(Immunofluorescence staining of CFL2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CFL2 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)

IHC (Immunohistochemisry)

(Immunochemical staining CFL2 in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohiostchemistry)

(Immunochemical staining CFL2 in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

WB (Western Blot)

(Anti-CFL2 rabbit polyclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLane B: Hela Whole Cell LysateLane C: NCI-H1299 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:18 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-CFL2 antibody

Cofilin 2 (muscle), also known as CFL2, is a member of cofilin family of the actin-binding protein superfamily. Cofilin2 shows significant homology to the other two members: cofilin 1 and DSTN, through its entire sequence, and contains residues conserved among the cofilin family that are responsible for actin-binding. Cofilin 2 (CFL2) is an important regulator of striated myocyte function. Purified cofilin 2 depolymerized actin filaments in a dose-and pH-dependent manner and reduced the apparent viscosity of an actin solution, although they did not co-sediment with actin filaments at all. Cofilin2 is not expressed in vegetative cells, but is transiently induced during the aggregation stage of development, whereas cofilin 1 was predominantly expressed in vegetative cells.

Product Categories/Family for anti-CFL2 antibody

Actin Dynamics Signaling Pathway

References

Aizawa H, et al. (2001) Cofilin-2, a novel type of cofilin, is expressed specifically at aggregation stage of Dictyostelium discoideum development. Genes Cells. 6 (10): 913-21.Papalouka V, et al. (2009) Muscle LIM protein interacts with cofilin 2 and regulates F-actin dynamics in cardiac and skeletal muscle. Papalouka V, Mol Cell Biol. 29 (22): 6046-58.

NCBI and Uniprot Product Information

• NCBI GI #: 343887344
• NCBI GeneID: 1073
• NCBI Accession #: NP_001230574.1
• NCBI GenBank Nucleotide #: NM_001243645.1
• UniProt Accession #: Q9Y281; G3V5P4
• Molecular Weight: 18,737 Da
• NCBI Official Full Name: cofilin-2 isoform 2
• NCBI Official Synonym Full Names: cofilin 2 (muscle)
• NCBI Official Symbol: CFL2
• NCBI Official Synonym Symbols: NEM7
• NCBI Protein Information: cofilin-2; cofilin, muscle isoform; nemaline myopathy type 7
• UniProt Protein Name: Cofilin-2
• UniProt Gene Name: CFL2
• UniProt Entry Name: COF2_HUMAN