IHC (Immunohistochemisry)
(Collagen I was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- Collagen I Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)
Rabbit Collagen Polyclonal Antibody | anti-COL1A1 antibody
Anti-Collagen I Antibody
Gene Names
Col1a1; Cola1; Mov13; Cola-1; Mov-13; Col1a-1
Reactivity
Mouse, RatNo cross reactivity with other proteins.
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Collagen, Antibody; Anti-Collagen I Antibody; Alpha 1 type I collagen; Alpha 2 type I collagen; Alpha-1 type I collagen; CO1A1; COL1A1; Collagen alpha-1 chain; Collagen alpha1(I) chain; Collagen alpha-1(I) chain; Collagen I; Collagen Type 1; Collagen type I alpha 1; Collagen type I alpha 2; collagen, type I, alpha 1; OI4; Pro alpha 1(I) collagen; Type I procollagen; P11087 ; anti-COL1A1 antibody
Host
Rabbit
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
1453
Applicable Applications for anti-COL1A1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I (1185-1207aa YDFSFLPQPPQEKSQDGGRYYRA), different from the related human sequence by three amino acids, and identical to the related rat sequence.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemisry)
(Collagen I was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- Collagen I Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohistochemisry)
(Collagen I was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- Collagen I Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(Collagen I was detected in paraffin-embedded sections of mouse lung tissues using rabbit anti- Collagen I Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(Collagen I was detected in paraffin-embedded sections of mouse lung tissues using rabbit anti- Collagen I Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
WB (Western Blot)
(Western blot analysis of Collagen I expression in mouse kidney extract (lane 1) and mouse cardiac muscle extract (lane 2). Collagen I at 180KD was detected using rabbit anti-Collagen I Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)
WB (Western Blot)
(Western blot analysis of Collagen I expression in mouse kidney extract (lane 1) and mouse cardiac muscle extract (lane 2). Collagen I at 180KD was detected using rabbit anti-Collagen I Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Related Product Information for anti-COL1A1 antibody
Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
References
1. Aihara, M., Lindsey, J. D., Weinreb, R. N. Ocular hypertension in mice with a targeted type I collagen mutation. Invest. Ophthal. Vis. Sci. 44: 1581-1585, 2003.
2. Cabral, W. A., Makareeva, E., Colige, A., Letocha, A. D., Ty, J. M., Yeowell, H. N., Pals, G., Leikin, S., Marini, J. C. Mutations near amino end of alpha-1(I) collagen cause combined osteogenesis imperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing. J. Biol. Chem. 280: 19259-19269, 2005.
2. Cabral, W. A., Makareeva, E., Colige, A., Letocha, A. D., Ty, J. M., Yeowell, H. N., Pals, G., Leikin, S., Marini, J. C. Mutations near amino end of alpha-1(I) collagen cause combined osteogenesis imperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing. J. Biol. Chem. 280: 19259-19269, 2005.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
117,820 Da
NCBI Official Full Name
collagen alpha-1(I) chain preproprotein
NCBI Official Synonym Full Names
collagen, type I, alpha 1
NCBI Official Symbol
Col1a1
NCBI Official Synonym Symbols
Cola1; Mov13; Cola-1; Mov-13; Col1a-1
NCBI Protein Information
collagen alpha-1(I) chain
UniProt Protein Name
Collagen alpha-1(I) chain
UniProt Gene Name
Col1a1
UniProt Synonym Gene Names
Cola1
UniProt Entry Name
CO1A1_MOUSE
Similar Products
Product Notes
The COL1A1 col1a1 (Catalog #AAA46614) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Collagen I Antibody reacts with Mouse, Rat No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the COL1A1 col1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.