Application Data
(Anti-Collagen III Picoband antibody, -2.jpgAll lanes: Anti Collagen III at 0.5ug/mlLane 1: MCF-4 Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 139KDObserved bind size: 139KD)
anti-Human Collagen III Polyclonal Antibody | anti-COL3A1 antibody
Anti-Collagen III Antibody
Gene Names
COL3A1; EDS4A
Reactivity
Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Collagen III, Antibody; Anti-Collagen III Antibody; Collagen alpha-1(III) chain; CO3A1_HUMAN; COL 3A1; COL3A1; Collagen alpha 1(III) chain; Collagen III alpha 1 chain precursor; Collagen III alpha 1 polypeptide; Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant); Collagen type III alpha 1; Collagen type III alpha; EDS4A; Ehlers Danlos syndrome type IV, autosomal dominant; Fetal collagen; Type III collagen; collagen, type III, alpha 1; anti-COL3A1 antibody
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Sequence Length
1466
Applicable Applications for anti-COL3A1 antibody
WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen III(1200-1221aa VGAAAIAGIGGEKAGGFAPYYG), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Application Data
(Anti-Collagen III Picoband antibody, -2.jpgAll lanes: Anti Collagen III at 0.5ug/mlLane 1: MCF-4 Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 139KDObserved bind size: 139KD)
Application Data
(Anti-Collagen III Picoband antibody, -2.jpgAll lanes: Anti Collagen III at 0.5ug/mlLane 1: MCF-4 Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 139KDObserved bind size: 139KD)
WB (Western Blot)
(Anti-Collagen III Picoband antibody, -1.jpgAll lanes: Anti Collagen III at 0.5ug/mlWB: Recombinant Human Collagen III Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
WB (Western Blot)
(Anti-Collagen III Picoband antibody, -1.jpgAll lanes: Anti Collagen III at 0.5ug/mlWB: Recombinant Human Collagen III Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Related Product Information for anti-COL3A1 antibody
Description: Rabbit IgG polyclonal antibody for Collagen alpha-1(III) chain(COL3A1) detection. Tested with WB in Human.
Background: COL3A1, also called EDS4A or Collagen alpha-1(III), is a protein that in humans is encoded by the COL3A1 gene, it is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1(III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it Is the major ligand of GPR56 in the developing brain.COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12
Background: COL3A1, also called EDS4A or Collagen alpha-1(III), is a protein that in humans is encoded by the COL3A1 gene, it is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1(III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it Is the major ligand of GPR56 in the developing brain.COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12
References
1. Janeczko RA, Ramirez F (Oct 1989). "Nucleotide and amino acid sequences of the entire human alpha 1 (III) collagen". Nucleic Acids Res 17 (16): 6742. 2. Superti-Furga A, Gugler E, Gitzelmann R, Steinmann B (Jun 1988). "Ehlers-Danlos syndrome type IV: a multi-exon deletion in one of the two COL3A1 alleles affecting structure, stability, and processing of type III procollagen". J Biol Chem 263 (13): 6226-32.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
111,907 Da
NCBI Official Full Name
collagen alpha-1(III) chain preproprotein
NCBI Official Synonym Full Names
collagen type III alpha 1
NCBI Official Symbol
COL3A1
NCBI Official Synonym Symbols
EDS4A
NCBI Protein Information
collagen alpha-1(III) chain
UniProt Protein Name
Collagen alpha-1(III) chain
UniProt Gene Name
COL3A1
UniProt Entry Name
CO3A1_HUMAN
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Product Notes
The COL3A1 col3a1 (Catalog #AAA46297) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Collagen III Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen III can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the COL3A1 col3a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen III, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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