Rabbit Collagen III Polyclonal Antibody

Collagen III

Cat. Num. AAA268982

• Reactivity: Human,Mouse,Rat,Cow
• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Purification method: Protein A Affinity Plank.
• Synonyms: Collagen III, Antibody; Collagen III; COL 3A1; COL3A1; Collagen alpha 1 (III) chain; Collagen III alpha 1 chain precursor; Collagen III alpha 1 polypeptide; Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant); Collagen type III alpha 1; Collagen type III alpha; EDS4A; Ehlers Danlos syndrome type IV; autosomal dominant; Fetal collagen; Type III collagen; CO3A1_HUMAN; Collagen alpha-1 (III) chain; type III preprocollagen alpha 1 chain.; anti-Collagen III antibody

• Host: Rabbit
• Reactivity: Human,Mouse,Rat,Cow
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purification method: Protein A Affinity Plank.
• Form/Format: Liquid; 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Concentration: 1 mg/mL. (varies by lot)

• Applicable Applications for anti-Collagen III antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: KLH conjugated synthetic peptide derived from human Collagen alpha 1 (III) chain 801-900/1466
• Subcellular localization: Secreted, Extracellular space, Extracellular matrix
• Conjugation: Biotin
• Preparation and Storage: Store at -20 degree C for one year. Avoid repeated freeze/thaw cycles.; The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 degree C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 degree C.

IHC (Immunohiostchemistry)

(Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Incubation: Anti-Collagen III Polyclonal Antibody, Unconjugated (AAA268982) 1:500, overnight at 4 ? , followed by conjugation to the secondary antibody and DAB staining.)

WB (Western Blot)

(Sample:Hela Cell (Human) Lysate at 40 ugPrimary: Anti- Collagen III (AAA268982) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 130 kD.)

Related Product Information for anti-Collagen III antibody

Description: Type III Collagen is a homotrimer, or a protein composed of three identical peptide chains (monomers), each called an alpha 1 chain of type III collagen. Formally, the monomers are called collagen type III, alpha-1 chain and in humans are encoded by the COL3A1 gene. Type III collagen is one of the fibrillar collagens whose proteins have a long, inflexible, triple-helical domain. In addition to being an integral structural component of many organs, type III collagen is also an important regulator of the diameter of type I and II collagen fibrils. Type III collagen is also known to facilitate platelet aggregation through its binding to platelets and therefore, play an important role in blood clotting. Type III collagen is found as a major structural component in hollow organs such as large blood vessels, uterus and bowel. It is also found in many other tissues together with type I collagen.

NCBI and Uniprot Product Information

• UniProt Accession #: P02461
• Molecular Weight: 117 KDa