WB (Western Blot)
(Detection of mouse COP1/RFWD2 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COP1/RFWD2 antibody (AAA211201 lot 4) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse COP1/RFWD2 Polyclonal Antibody | anti-COP1/RFWD2 antibody
Rabbit anti-COP1/RFWD2 Antibody, Affinity Purified
Gene Names
RFWD2; COP1; RNF200
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
COP1/RFWD2, Antibody; Rabbit anti-COP1/RFWD2 Antibody, Affinity Purified; RFWD2; RING-type E3 ubiquitin transferase RFWD2; RNF200; RING finger protein 200; RING finger and WD repeat domain protein 2; ring finger and WD repeat domain 2, E3 ubiquitin protein ligase; hCOP1; E3 ubiquitin-protein ligase RFWD2; E3 ubiquitin-protein ligase COP1; constitutive photomorphogenic protein 1; constitutive photomorphogenesis protein 1 homolog; putative ubiquitin ligase COP1; COP1, E3 ubiquitin ligase; anti-COP1/RFWD2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-COP1/RFWD2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse COP1/RFWD2 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COP1/RFWD2 antibody (AAA211201 lot 4) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse COP1/RFWD2 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COP1/RFWD2 antibody (AAA211201 lot 4) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human COP1/RFWD2 by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, U2OS, K-562, HT-29, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COP1/RFWD2 antibody (AAA211201 lot 4) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human COP1/RFWD2 by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, U2OS, K-562, HT-29, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COP1/RFWD2 antibody (AAA211201 lot 4) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human COP1/RFWD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COP1/RFWD2 antibody (AAA211201 lot 4) used for IP at 6 ug per reaction. COP1/RFWD2 was also immunoprecipitated by a previous lot of this antibody (AAA211201 lot 3) and a second antibody against a different epitope of COP1/RFWD2 . For blotting immunoprecipitated COP1/RFWD2, AAA211201 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human COP1/RFWD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COP1/RFWD2 antibody (AAA211201 lot 4) used for IP at 6 ug per reaction. COP1/RFWD2 was also immunoprecipitated by a previous lot of this antibody (AAA211201 lot 3) and a second antibody against a different epitope of COP1/RFWD2 . For blotting immunoprecipitated COP1/RFWD2, AAA211201 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-COP1/RFWD2 antibody
Constitutive photomorphogenic protein 1 (COP1) has been characterized in plants as a RING-finger-containing protein that functions to repress plant photomorphogenesis. In mammals, COP1 has been identified as an E3 ubiquitin ligase that negatively regulates p53 protein levels in unstressed cells. In response to DNA damage COP1 is phosphorylated by the ataxia telangiectasia mutated (ATM) protein kinase and is rapidly degraded releasing p53 to exert its tumor suppressor activities.
Product Categories/Family for anti-COP1/RFWD2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,914 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase RFWD2 isoform a
NCBI Official Synonym Full Names
ring finger and WD repeat domain 2
NCBI Official Symbol
RFWD2
NCBI Official Synonym Symbols
COP1; RNF200
NCBI Protein Information
E3 ubiquitin-protein ligase RFWD2
UniProt Protein Name
E3 ubiquitin-protein ligase RFWD2
UniProt Gene Name
RFWD2
UniProt Synonym Gene Names
COP1; RNF200; hCOP1
UniProt Entry Name
RFWD2_HUMAN
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Product Notes
The COP1/RFWD2 rfwd2 (Catalog #AAA211201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-COP1/RFWD2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's COP1/RFWD2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the COP1/RFWD2 rfwd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COP1/RFWD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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