Rabbit Creatine kinase Polyclonal Antibody | anti-CKM antibody

Creatine kinase antibody

Cat. Num. AAA107558

• Gene Names: CKM; CKMM; M-CK
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Creatine kinase antibody was purified by antigen-affinity chromatography
• Synonyms: Creatine kinase, Antibody; Creatine kinase antibody; Polyclonal Creatine kinase; Anti-Creatine kinase; creatine kinase B chain; CKBB; B-CK; creatine kinase-B; CKB; creatine kinase B-type; anti-CKM antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Creatine kinase antibody was purified by antigen-affinity chromatography
• Form/Format: Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
• Concentration: 0.54 mg/ml (varies by lot)
• Sequence Length: 381

• Applicable Applications for anti-CKM antibody: WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence)
• Biological Significance: The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
• Cross-Reactivity: Human, Mouse
• Immunogen: Creatine kinase antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 165 and 381 of Creatine kinase
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

IHC (Immunohistochemistry)

(Immunohistochemical staining of paraffin-embedded HSC3 xenograft using Creatine kinase antibody at a dilution of 1:100)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Creatine Kinase BB antibody at 1:100 dilution.)

WB (Western Blot)

(Western blot analysis of 30 ug of whole cell lysate (A:H1299; B:HeLa S3) using a 12 % SDS PAGE gel and Creatine kinase antibody at a dilution of 1:1000)

WB (Western Blot)

(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10 % SDS PAGE gel and Creatine kinase antibody at a dilution of 1:10000)

Related Product Information for anti-CKM antibody

Affinity purified Rabbit polyclonal Creatine kinase antibody

Product Categories/Family for anti-CKM antibody

Proteases; Inhibitors; & Enzymes; Purified Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 180588
• NCBI GeneID: 1158
• UniProt Accession #: P06732; Q96QL9
• Molecular Weight: 43 kDa
• NCBI Official Full Name: creatine kinase
• NCBI Official Synonym Full Names: creatine kinase, muscle
• NCBI Official Symbol: CKM
• NCBI Official Synonym Symbols: CKMM; M-CK
• NCBI Protein Information: creatine kinase M-type
• UniProt Protein Name: Creatine kinase M-type
• UniProt Gene Name: CKM
• UniProt Synonym Gene Names: CKMM
• UniProt Entry Name: KCRM_HUMAN